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Literature DB >> 7129599

A rapid quantitative assay for activated neutrophils.

Abstract

We present here a rapid, sensitive, and convenient assay for activated human PMNs based on detecting the decreased optical density (OD) of aggregated cell suspensions. This quantitative assay uses an ELIZA machine to measure OD changes, with time, of activated cells (5 X 10(5) cells/well) in microtiter plates. The assay is sensitive, detecting aggregation induced by as little as 0.0001 microgram/ml of LPS, or lymphokines in Con-A-activated supernatant diluted 1/500. The assay permits analysis of 400 separate PMN suspensions on the same day starting with less than 80 ml of blood.

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Year: 1982 PMID： 7129599 DOI： 10.1007/bf00916411

Source DB: PubMed Journal: Inflammation ISSN： 0360-3997 Impact factor: 4.092

10 in total

1. Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia.

Authors: P R Craddock; D Hammerschmidt; J G White; A P Dalmosso; H S Jacob
Journal: J Clin Invest Date: 1977-07 Impact factor: 14.808

2. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors: A Böyum
Journal: Scand J Clin Lab Invest Suppl Date: 1968

3. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system.

Authors: R R MacGregor; P J Spagnuolo; A L Lentnek
Journal: N Engl J Med Date: 1974-09-26 Impact factor: 91.245

4. Induction of a transient elevation in intracellular levels of adenosine-3',5'-cyclic monophosphate by chemotactic factors: an early event in human neutrophil activation.

Authors: L Simchowitz; L C Fischbein; I Spilberg; J P Atkinson
Journal: J Immunol Date: 1980-03 Impact factor: 5.422

5. Digital integration of granulocyte aggregation responses. A simple and reproducible method for the quantitation of granulocyte adhesiveness.

Authors: P R Craddock; J G White; D J Weisdorf; D E Hammerschmidt
Journal: Inflammation Date: 1980-12 Impact factor: 4.092

6. Concanavalin A-induced macrophage aggregating factor.

Authors: P Badenoch-Jones
Journal: Aust J Exp Biol Med Sci Date: 1981-10

7. Effects of the numbers and sizes of platelet aggregates on the optical density of plasma.

Authors: G V Born; M Hume
Journal: Nature Date: 1967-09-02 Impact factor: 49.962

8. Corticosteroids inhibit complement-induced granulocyte aggregation. A possible mechanism for their efficacy in shock states.

Authors: D E Hammerschmidt; J G White; P R Craddock; H S Jacob
Journal: J Clin Invest Date: 1979-04 Impact factor: 14.808

9. Properties of concanavalin A-elicited granule exocytosis from human polymorphonuclear neutrophils.

Authors: R J Smith; S S Iden
Journal: Inflammation Date: 1980-12 Impact factor: 4.092

10. Involvement of bivalent cations and arachidonic acid in neutrophil aggregation.

Authors: J T O'Flaherty
Journal: Inflammation Date: 1980-06 Impact factor: 4.092

10 in total

8 in total

A rapid quantitative assay for activated neutrophils.

1. Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia.

2. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

3. Inhibition of granulocyte adherence by ethanol, prednisone, and aspirin, measured with an assay system.

4. Induction of a transient elevation in intracellular levels of adenosine-3',5'-cyclic monophosphate by chemotactic factors: an early event in human neutrophil activation.

5. Digital integration of granulocyte aggregation responses. A simple and reproducible method for the quantitation of granulocyte adhesiveness.

6. Concanavalin A-induced macrophage aggregating factor.

7. Effects of the numbers and sizes of platelet aggregates on the optical density of plasma.

8. Corticosteroids inhibit complement-induced granulocyte aggregation. A possible mechanism for their efficacy in shock states.

9. Properties of concanavalin A-elicited granule exocytosis from human polymorphonuclear neutrophils.

10. Involvement of bivalent cations and arachidonic acid in neutrophil aggregation.

1. Role of extracellular calcium in neutrophil responsiveness to chemotactic tripeptides.

2. An enzyme-assessed microplate-assay for neutrophil adherence. I. IgA-induced adherence of human PMNs.

3. Effect of fluoride on movement of concanavalin A-acceptor molecules of human neutrophils.

4. Fluoride activation of neutrophils: similarities to formylmethionyl-leucyl-phenylalanine.

5. Phorbol myristate acetate induction of chemotactic migration of human polymorphonuclear neutrophils.

6. Endogenous component chemotactic assay (ECCA).

7. Aggregated human colostral sIgA stimulates delayed, non-complement-dependent, NBT reduction by human neutrophils.

8. Activated rat neutrophils. A sequential quantitative assay for aggregation and NBT reduction.