| Literature DB >> 7117742 |
M Hiratsuka, T Senoo, T Kimoto, M Namba.
Abstract
An improved short-term culture method for human mammary epithelial cells was developed and the details are presented. Briefly, after the digestion of biopsy tissues with 0.2% collagenase solution for 16 to 18 hr, 4 consecutive fractions were obtained by differential sedimentation. The last fraction, S-4, contained organoids having alveolar- or duct-like structures and gave rise to mammary epithelial cell growth with minimal contamination of fibroblasts.Entities:
Mesh：
Year: 1982 PMID： 7117742
Source DB: PubMed Journal: Gan ISSN： 0016-450X