The excretion of metabolites of the D-glucuronic acid pathway in human urine. Effect of phenobarbitone administration.

1. The urinary excretion of four metabolites of the D-glucuronic acid pathway, namely D-glucaric acid, free (unconjugated) D-glucuronic acid, L-gulonic acid and xylitol, has been studied in normal male volunteers and in male and female epileptic patients receiving phenobarbitone anticonvulsant therapy. 2. In normal male subjects the urinary excretion of the D-glucuronic acid metabolites, expressed per unit of creatinine, was similar in first void and total (24 h) urine samples and was fairly constant over a period of 4 weeks. 3. In male and female epileptic patients phenobarbitone treatment enhanced the urinary excretion of D-glucaric acid and xylitol and the combined excretion of D-glucaric acid, l-gulonic acid and xylitol. 4. It is suggested that the measurement of a spectrum of urinary D-glucuronic acid metabolites may provide a more reliable index for assessment of the induction of hepatic xenobiotic-metabolizing enzyme activities in man than the determination of urinary D-glucaric acid alone.