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Literature DB >> 7109030

In vitro packaging of bacteriophage T7 DNA requires ATP.

Abstract

Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.

Entities: Chemical Species

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Year: 1982 PMID： 7109030 PMCID： PMC256132 DOI： 10.1128/JVI.43.1.365-367.1982

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

21 in total

1. Genetic recombination of bacteriophage T7 DNA in vitro.

Authors: P D Sadowski; D Vetter
Journal: Proc Natl Acad Sci U S A Date: 1976-03 Impact factor: 11.205

Review 2. Bacteriophage T7.

Authors: F W Studier
Journal: Science Date: 1972-04-28 Impact factor: 47.728

8. Replication of duplex DNA by bacteriophage T7 DNA polymerase and gene 4 protein is accompanied by hydrolysis of nucleoside 5'-triphosphates.

Authors: R Kolodner; C C Richardson
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

In vitro packaging of bacteriophage T7 DNA requires ATP.

1. Genetic recombination of bacteriophage T7 DNA in vitro.

Review 2. Bacteriophage T7.

3. The enzymatic repair of DNA, II. Characterization of phage-induced sealase.

4. The genetics and physiology of bacteriophage T7.

5. Bacteriophage T7 morphogenesis: phage-related particles in cells infected with wild-type and mutant T7 phage.

6. A mutant of bacteriophage T7 deficient in polynucleotide ligase.

7. In vitro packaging of phage T3 DNA.

8. Replication of duplex DNA by bacteriophage T7 DNA polymerase and gene 4 protein is accompanied by hydrolysis of nucleoside 5'-triphosphates.

9. Packaging and maturation of DNA of bacteriophage T7 in vitro.

10. In vitro packaging of UV radiation-damaged DNA from bacteriophage T7.

1. In vitro repair of double-strand breaks accompanied by recombination in bacteriophage T7 DNA.

2. In vitro repair of gaps in bacteriophage T7 DNA.

3. Bacteriophage P22 in vitro DNA packaging monitored by agarose gel electrophoresis: rate of DNA entry into capsids.