Literature DB >> 7107860

Development of an enzyme-linked immunosorbent assay for studying Vibrio cholerae cell surface antigens.

S J Cryz, E Fürer, R Germanier.   

Abstract

An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed against lipopolysaccharide and non-lipopolysaccharide surface antigens. In addition, the CSA ELISA was capable of detecting non-vibriocidal antibody. An antiserum raised in rabbits by immunization with live V. cholerae 1418 (Ogawa, El Tor) was capable of reacting with various heterologous strains of V. cholerae used as immobilized antigens. Therefore, common antigens shared by V. cholerae strains could be detected by using the CSA ELISA.

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Year:  1982        PMID： 7107860      PMCID： PMC272291          DOI： 10.1128/jcm.16.1.41-45.1982

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Response of man to infection with Vibrio cholerae. II. Protection from illness afforded by previous disease and vaccine.

Authors:  R A Cash; S I Music; J P Libonati; J P Craig; N F Pierce; R B Hornick
Journal:  J Infect Dis       Date:  1974-10       Impact factor: 5.226

2.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

3.  Quantitative assay of a protein antigen of vibrio cholerae involved in the vibriocidal action of antibody and complement.

Authors:  S H Neoh; D Rowley
Journal:  Aust J Exp Biol Med Sci       Date:  1971-12

4.  Enzyme-linked immunosorbent assays for cholera serology.

Authors:  J Holmgren; A M Svennerholm
Journal:  Infect Immun       Date:  1973-05       Impact factor: 3.441

5.  Survival and multiplication of Vibrio cholerae in the upper bowel of infant mice.

Authors:  V S Baselski; R A Medina; C D Parker
Journal:  Infect Immun       Date:  1978-11       Impact factor: 3.441

6.  Vibriocidal and agglutinating antibody patterns in cholera patients.

Authors:  R B Sack; D Barua; R Saxena; C C Carpenter
Journal:  J Infect Dis       Date:  1966-12       Impact factor: 5.226

7.  Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies.

Authors:  E T Nelson; J D Clements; R A Finkelstein
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

8.  Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.

Authors:  J T Kelley; C D Parker
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

9.  Field trials of monovalent Ogawa and Inaba cholera vaccines in rural Bangladesh--three years of observation.

Authors:  W H Mosley; K M Aziz; A S Rahman; A K Chowdhury; A Ahmed
Journal:  Bull World Health Organ       Date:  1973       Impact factor: 9.408

10.  Report of the 1966-67 cholera vaccine field trial in rural East Pakistan. 3. The lack of effect of prior vaccination or circulating vibriocidal antibody on the severity of clinical cholera.

Authors:  W M McCormack; A S Rahman; A K Chowdhury; W H Mosley; R A Phillips
Journal:  Bull World Health Organ       Date:  1969       Impact factor: 9.408
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  7 in total

1.  Flow cytometric analysis for adhesion of Vibrio cholerae to human intestinal epithelial cell.

Authors:  H Taguchi; H Yamaguchi; T Y Osaki; T Yamamoto; S Ogata; S Kamiya
Journal:  Eur J Epidemiol       Date:  1997-09       Impact factor: 8.082

2.  Volunteer studies of deletion mutants of Vibrio cholerae O1 prepared by recombinant techniques.

Authors:  M M Levine; J B Kaper; D Herrington; G Losonsky; J G Morris; M L Clements; R E Black; B Tall; R Hall
Journal:  Infect Immun       Date:  1988-01       Impact factor: 3.441

3.  Serum antibody responses of divers to waterborne pathogens.

Authors:  G A Losonsky; J A Hasan; A Huq; S Kaintuck; R R Colwell
Journal:  Clin Diagn Lab Immunol       Date:  1994-03

4.  Evaluation of the human immune response to outer membrane proteins of Vibrio cholerae.

Authors:  S D Sears; K Richardson; C Young; C D Parker; M M Levine
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

5.  Effect of chemical and heat inactivation on the antigenicity and immunogenicity of Vibrio cholerae.

Authors:  S J Cryz; E Fürer; R Germanier
Journal:  Infect Immun       Date:  1982-10       Impact factor: 3.441

6.  Major outer membrane proteins of Vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization.

Authors:  D K Sengupta; T K Sengupta; A C Ghose
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441

7.  Development of an enzyme-linked immunosorbent assay for studying Pseudomonas aeruginosa cell surface antigens.

Authors:  R S Borowski; L M Stock; N L Schiller
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948
  7 in total

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