Literature DB >> 7092827

The subcellular distribution of rat liver serine-pyruvate aminotransferase.

K V Rowsell, L M Al-Naama, P Benett.   

Abstract

1. The subcellular distribution of L-serine-pyruvate aminotransferase activity in rat liver was investigated. About 80% was recovered from cell-free homogenates in a 'total-particles' fraction and the remainder in the cytosol. 2. Subfractionation of the particles by differential sedimentation and on sucrose density gradients showed a distribution for serine-pyruvate aminotransferase activity closely matching that observed for mitochondrial marker enzymes. 3. A study of the solubilization of enzymes from combined subcellular particles by digitonin at various concentrations also indicated a common subcellular location for serine-pyruvate aminotransferase and established mitochondrial enzymes. 4. The increase in liver serine-pyruvate amino-transferase activity induced by glucagon injection was accounted for as an increased mitochondrial activity.

Entities:  

Mesh：

Substances：

Year:  1982        PMID： 7092827      PMCID： PMC1158134          DOI： 10.1042/bj2020483

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

1.  The isolation of outer and inner mitochondrial membranes.

Authors:  J W Greenawalt
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

2.  Gluconeogenesis from serine by the serine-dehydratase-dependent pathway in rat liver.

Authors:  I V Sandoval; A Sols
Journal:  Eur J Biochem       Date:  1974-04-16

3.  Increased liver L-serine-pyruvate aminotransferase activity under gluconeogenic conditions.

Authors:  E V Rowsell; A H al-Tai; J A Carnie
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857

4.  The subcellular distribution of rat liver L-alanine-glyoxylate aminotransferase in relation to a pathway for glucose formation involving glyoxylate.

Authors:  E V Rowsell; K Snell; J A Carnie; K V Rowsell
Journal:  Biochem J       Date:  1972-03       Impact factor: 3.857

5.  Liver-L-alanine-glyoxylate and L-serine-pyruvate aminotransferase activities: an apparent association with gluconeogenesis.

Authors:  E V Rowsell; K Snell; J A Carnie; A H Al-Tai
Journal:  Biochem J       Date:  1969-12       Impact factor: 3.857

Review 6.  Peroxisomes (microbodies and related particles).

Authors:  C De Duve; P Baudhuin
Journal:  Physiol Rev       Date:  1966-04       Impact factor: 37.312

7.  The submitochondrial localization of monoamine oxidase. An enzymatic marker for the outer membrane of rat liver mitochondria.

Authors:  C Schnaitman; V G Erwin; J W Greenawalt
Journal:  J Cell Biol       Date:  1967-03       Impact factor: 10.539

8.  The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.

Authors:  F Leighton; B Poole; H Beaufay; P Baudhuin; J W Coffey; S Fowler; C De Duve
Journal:  J Cell Biol       Date:  1968-05       Impact factor: 10.539

9.  Separation of mitochondrial membranes of Neurospora crassa. II. Submitochondrial localization of the isoleucine-valine biosynthetic pathway.

Authors:  W E Cassady; E H Leiter; A Bergquist; R P Wagner
Journal:  J Cell Biol       Date:  1972-04       Impact factor: 10.539

10.  Enzymatic properties of the inner and outer membranes of rat liver mitochondria.

Authors:  C Schnaitman; J W Greenawalt
Journal:  J Cell Biol       Date:  1968-07       Impact factor: 8.077
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.