Literature DB >> 7088686

Limiting section thickness of guinea pig olfactory cortical slices studied from tissue pO2 values and electrical activities.

T Fujii, H Baumgärtl, D W Lübbers.   

Abstract

The relationship between tissue oxygen partial pressure (pO2) values and electrical activities of guinea pig olfactory cortical slices were investigated as the slices were superfused with Krebs-Ringer's solution equilibrated with different gas mixtures. The pO2 values were measured in the slices with oxygen microelectrodes (tip diameter less than 1 micrometer). 1. Studies of pO2 measurements showed the variability of minimum pO2 value of oxygen profiles in the tissue slice. The profile depends on the pO2 value of the superfusate and on the thickness and the oxygen consumption of the slice. With our experimental conditions an anoxic area developed in the middle layers of the slice when the thickness of the slice exceeded ca. 430 micrometers; in thinner slices there was no anoxic area. In our case the limiting section thickness of the slice was ca.430 micrometers from the viewpoint of tissue pO2 value. 2. The N potential (extra-cellularly recorded EPSP) showed a tendency to decrease in amplitude, for slices being thicker than ca 430 micrometers. It would seem reasonable to think that the decrement of the N potential was brought about by the existence of the anoxic area. 3. When the slice was bubbled with 25, 45 or 95% O2, the tissue pO2 value changed, and the N potential height also changed. The N potential was higher in amplitude when bubbled with 95% O2 than with 25% O2. On the other hand, the amplitude of the IS potential (the lateral olfactory tract potential) was not influenced as much as that of the N potential by the change of tissue pO2 value in the slice. 4. The tissue pO2 value was continuously measured during the electrical stimulation of the lateral olfactory tract. The steady state level of tissue pO2 value obtained during the stimulation diminished as the frequency of stimulation increased from 5-30 Hz.

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Year:  1982        PMID: 7088686     DOI: 10.1007/BF00582396

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN: 0031-6768            Impact factor:   3.657


  15 in total

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Authors:  J FIELD
Journal:  Methods Med Res       Date:  1948

2.  The production of persistent changes in the level of neuronal activity by brief local cooling of the cerebral cortex of the rat.

Authors:  I B Gartside; O C Lippold
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3.  Effects of breathing O 2 or O 2 +CO 2 and of the injection of neurohumors on the PO 2 of cat cerebral cortex.

Authors:  W J Whalen; R Ganfield; P Nair
Journal:  Stroke       Date:  1970 May-Jun       Impact factor: 7.914

4.  Measurements of oxygen tension in tissues with a micro oxygen electrode.

Authors:  W J Whalen; P Nair; R A Ganfield
Journal:  Microvasc Res       Date:  1973-05       Impact factor: 3.514

5.  Electrical activity observed in guinea-pig olfactory cortex maintained in vitro.

Authors:  C D Richards; R Sercombe
Journal:  J Physiol       Date:  1968-08       Impact factor: 5.182

6.  Structure of cat frontal olfactory cortex.

Authors:  C F Stevens
Journal:  J Neurophysiol       Date:  1969-03       Impact factor: 2.714

7.  Gas-blow and medium-flow method for stable recording of electrical activities in the sliced mammalian cerebral tissue in vitro.

Authors:  T Fujii; S Kubo; Y Iwase
Journal:  Nihon Seirigaku Zasshi       Date:  1970

8.  The postnatal development of cortical structures and electrical activities in the guinea pig olfactory cortex slice.

Authors:  T Fujii; K Murayama; Y Ibata
Journal:  Brain Res       Date:  1978-03-10       Impact factor: 3.252

9.  Activation energy in the mammalian brain slice as determined by oxygen micro-electrode measurements.

Authors:  T Fujii; D G Buerk; W J Whalen
Journal:  Jpn J Physiol       Date:  1981

10.  Oxygen and vascular smooth muscle contraction revisited.

Authors:  A E Chang; R Detar
Journal:  Am J Physiol       Date:  1980-05
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  12 in total

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6.  Fictive respiratory rhythm in the isolated brainstem of frogs.

Authors:  H A McLean; N Kimura; N Kogo; S F Perry; J E Remmers
Journal:  J Comp Physiol A       Date:  1995-05       Impact factor: 1.836

7.  Microenvironment of respiratory neurons in the in vitro brainstem-spinal cord of neonatal rats.

Authors:  J Brockhaus; K Ballanyi; J C Smith; D W Richter
Journal:  J Physiol       Date:  1993-03       Impact factor: 5.182

8.  Interstitial PCO2 and pH in rat hippocampal slices measured by means of a novel fast CO2/H(+)-sensitive microelectrode based on a PVC-gelled membrane.

Authors:  J Voipio; K Kaila
Journal:  Pflugers Arch       Date:  1993-05       Impact factor: 3.657

9.  P/Q Ca2+ channel blockade stops spreading depression and related pyramidal neuronal Ca2+ rise in hippocampal organ culture.

Authors:  Phillip E Kunkler; Richard P Kraig
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10.  Quantitative effects of some muscarinic agonists on evoked surface-negative field potentials recorded from the guinea-pig olfactory cortex slice.

Authors:  S H Williams; A Constanti
Journal:  Br J Pharmacol       Date:  1988-04       Impact factor: 8.739

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