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Literature DB >> 7087961

Evidence of the mannosylation of a non-histone protein in monkey liver chromatin.

Abstract

Mannose is incorporated in monkey liver chromatin by the means of a nuclear membrane mannosyltransferase. 14C-labelled chromatin is dissociated either by sulfuric acid or 6 M urea and 0.4 M GuCl. The fractions then enriched in non-histone 14C-labelled proteins are excluded from Ultro-gel AcA 202, their analysis in SDS-polyacrylamide gel electrophoresis shows that radioactivity fits with one major protein band, confirming the presence of at least a non-histone protein labelled with mannose in monkey liver chromatin, with an apparent molecular weight of 13,000.

Entities: Chemical Disease

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Year: 1982 PMID： 7087961 DOI： 10.1007/BF00573844

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

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References

14 in total

9. Nematode chromosomal proteins--I. Isolation of chromatin and preliminary characterization of the chromosomal proteins of Caenorhabditis elegans.

Authors: J B Vanfleteren; K Neirynck; D Huylebroeck
Journal: Comp Biochem Physiol B Date: 1979

10. A non-histone chromatin protein that is a specific phosphate acceptor of nuclear cAMP-independent protein kinase from mouse spleen cells.

Authors: K Ohtsuki; H Shiraishi; E Yamada; M Nakamura; N Ishida
Journal: J Biol Chem Date: 1980-03-25 Impact factor: 5.157

Evidence of the mannosylation of a non-histone protein in monkey liver chromatin.

1. Are glycoproteins and glycosaminoglycans components of the eukaryotic genome?

2. Preparation of native chromatin and damage caused by shearing.

3. Lectins as probes of chromatin structure. Binding of concanavalin A to purified rat liver chromatin.

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7. [The presence of glycosyltransferases on chromatin acceptors in monkey liver nuclear membranes (author's transl)].

8. Characterization of mononucleosomes and associated glycoproteins from Ehrlich ascites tumour cells.

9. Nematode chromosomal proteins--I. Isolation of chromatin and preliminary characterization of the chromosomal proteins of Caenorhabditis elegans.

10. A non-histone chromatin protein that is a specific phosphate acceptor of nuclear cAMP-independent protein kinase from mouse spleen cells.