| Literature DB >> 708776 |
Abstract
Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. The purified rhodopsin (A280/A480 = 2.5) contained 2.3 mol of glucosamine and 1.2 mol of phospholipid per mol of rhodopsin. The photoproduct metarhodopsin was also stable in these detergent solutions as in digitonin solution. Concanavalin A had no affinity for retinochrome.Entities:
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Year: 1978 PMID: 708776 DOI: 10.1016/0005-2795(78)90053-3
Source DB: PubMed Journal: Biochim Biophys Acta ISSN: 0006-3002