High-performance liquid chromatographic analysis of hydrocortisone drug substance, tablets, and enema.

Methods for the analysis of hydrocortisone drug substance, tablets, and enema were developed using adsorption high-performance liquid chromatography (HPLC). This HPLC system was shown to be capable of isolating hydrocortisone from its degradation products, synthesis precursor, and related corticosteroids. The accuracy, precision, and linearity of the HPLC assay methods and their applicability to commercial products has been demonstrated.