| Literature DB >> 7082864 |
H Misumi, H Wada, Y Ichiba, T Shohmori, M Kosaka.
Abstract
Red blood cell lysates were applied to DEAE cellulose paper for detection of glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase (G-6-PD) was separated from 6-phosphogluconate dehydrogenase (6-PGD) by DEAE-cellulose paper-chromatography and their activities were detected on the chromatogram by applying the reaction mixture. The fluorescence of NADPH formed by G-6-PD was not interfered with by 6-PGD activity. This is a sensitive procedure which detects slight loss of G-6-PD in red blood cells and could be applied to the detection of G-6-PD deficiency.Entities:
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Year: 1982 PMID： 7082864 DOI： 10.1007/BF00320496
Source DB: PubMed Journal: Blut ISSN： 0006-5242