Literature DB >> 7080832

Detection of measles IgM antibodies by enzyme-linked immunosorbent assay (ELISA).

I R Pedersen, A Antonsdottir, T Evald, C H Mordhorst.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detectors of IgM antibodies in serum during acute or recent measles infections is described. False positive reactions due to rheumatoid factors (RF) were not observed in the test of human sera to which were added sera containing RF. Monospecific antisera against the haemagglutinin, nucleoprotein and polymerase of measles virus were used in a blocking IgM ELISA, revealing a sensitive method for the detection of the antibodies that take part in the immune reactions. An ELISA for the detection of measles-specific IgG in order to reveal IgM or IgG antibodies in the human sera is also described.

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Year:  1982        PMID： 7080832     DOI： 10.1111/j.1699-0463.1982.tb00098.x

Source DB:  PubMed          Journal:  Acta Pathol Microbiol Immunol Scand B        ISSN： 0108-0180


  10 in total

1.  Comparison of the MEASLESTAT M test kit with the sucrose density gradient centrifugation-hemagglutination inhibition method for detection of measles virus-specific immunoglobulin M.

Authors:  G S Withers; W I Sweimler; B Kleger
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948

2.  Infection studies in mink with seal-derived morbillivirus.

Authors:  M Blixenkrone-Møller; V Svansson; P Have; A Bøtner; J Nielsen
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

3.  Simultaneous primary infections with Epstein-Barr virus and measles virus in fatal acute encephalitis.

Authors:  M Melbye; P Ebbesen; N Jacobsen; C H Mordhorst
Journal:  Br Med J (Clin Res Ed)       Date:  1983-02-12

4.  Comparison of nonspecific reactivity in indirect and reverse immunoassays for measles and mumps immunoglobulin M antibodies.

Authors:  H Tuokko
Journal:  J Clin Microbiol       Date:  1984-11       Impact factor: 5.948

5.  Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles.

Authors:  A W Lievens; P A Brunell
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

6.  Assay of measles virus IgM and IgG class antibodies by use of peroxidase-labelled viral antigens.

Authors:  J Salonen; R Vainionpää; P Halonen
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

7.  Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus.

Authors:  D D Erdman; L J Anderson; D R Adams; J A Stewart; L E Markowitz; W J Bellini
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

8.  Detection of IgM antibodies to measles virus by enzyme-immunoassay.

Authors:  H Tuokko; A Salmi
Journal:  Med Microbiol Immunol       Date:  1983       Impact factor: 3.402

9.  Evaluation of hemagglutinin protein-specific immunoglobulin M for diagnosis of measles by an enzyme-linked immunosorbent assay based on recombinant protein produced in a high-efficiency mammalian expression system.

Authors:  F B Bouche; N H Brons; S Houard; F Schneider; C P Muller
Journal:  J Clin Microbiol       Date:  1998-12       Impact factor: 5.948

10.  Use of monoclonal antibodies to human immunoglobulin M in "capture" assays for measles and rubella immunoglobulin M.

Authors:  B Forghani; C K Myoraku; N J Schmidt
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948
  10 in total

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