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Literature DB >> 70785

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

Abstract

The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.

Entities: Chemical Species

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Year: 1977 PMID： 70785 PMCID： PMC431313 DOI： 10.1073/pnas.74.7.2840

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

Authors: F Sanger; A R Coulson
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

3. Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

Authors: K Horiuchi; N D Zinder
Journal: Proc Natl Acad Sci U S A Date: 1975-07 Impact factor: 11.205

Review 4. Restriction endonucleases in the analysis and restructuring of dna molecules.

Authors: D Nathans; H O Smith
Journal: Annu Rev Biochem Date: 1975 Impact factor: 23.643

5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors: J M Bailey; N Davidson
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

6. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis.

Authors: R V Guntaka; O C Richards; P R Shank; H J Kung; N Davidson
Journal: J Mol Biol Date: 1976-09-15 Impact factor: 5.469

7. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

8. Persistence of type 5 adenovirus DNA in cells transformed by temperature-sensitive mutant, H5ts125.

Authors: A J Mayer; H S Ginsberg
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

9. Effect of deoxyribonucleic acid length on the adenosine triphosphatase activity of Escherichia coli restriction endonuclease B.

Authors: K Horiuchi; G F Vovis; N D Zinder
Journal: J Biol Chem Date: 1974-01-25 Impact factor: 5.157

10. Cleavage map of linear mouse sarcoma virus DNA.

Authors: E Canaani; P Duesberg; D Dina
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

34 in total

1. Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

Authors: G F Gerard; D K Fox; M Nathan; J M D'Alessio
Journal: Mol Biotechnol Date: 1997-08 Impact factor: 2.695

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields.

1. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.

2. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

3. Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

Review 4. Restriction endonucleases in the analysis and restructuring of dna molecules.

5. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

6. Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis.

7. A new method for sequencing DNA.

8. Persistence of type 5 adenovirus DNA in cells transformed by temperature-sensitive mutant, H5ts125.

9. Effect of deoxyribonucleic acid length on the adenosine triphosphatase activity of Escherichia coli restriction endonuclease B.

10. Cleavage map of linear mouse sarcoma virus DNA.

1. Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

2. Assumed initiation site of fibroin gene transcription.

3. Strand-specific attachment of avidin-spheres to double-stranded poliovirus RNA.

4. Sequence complexity of cDNA transcribed from a diverse mRNA population.

5. RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.

6. Partial purification and characterization of the messenger RNA for cell fibronectin.

7. Complex population of mRNA sequences in large polyadenylylated nuclear RNA molecules.

8. In vitro synthesis of infectious transforming DNA by the avian sarcoma virus reverse transcriptase.

9. Selective amplification of mouse mammary tumor virus in mammary tumors of GR mice.

10. The role of template-primer in protection of reverse transcriptase from thermal inactivation.