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Literature DB >> 7075350

Isolation of DNA from single microsurgically excised bands of polytene chromosomes of Chironomus.

Abstract

A method is described for excising by a glass knife single bands of isolated polytene chromosomes of the salivary glands of Chironomus tentans larvae. DNA strands were isolated from cut-out bands and their contour lengths were determined on electron micrographs. The mean contour length of DNA strands isolated from the double band I-8A was about twice that of the single band I-11B, namely 63 versus 34 micrometers. The described method may be applicable for molecular studies on single bands (e.g., by DNA cloning).

Entities: Species

Mesh：

Substances：
DNA

Year: 1982 PMID： 7075350 DOI： 10.1007/bf00292850

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

19 in total

Review 1. Transcription in polytene chromosomes.

Authors: B Daneholt
Journal: Cell Date: 1975-01 Impact factor: 41.582

2. Quantitative analysis of DNA content in the salivary gland chromosomes of Chironomus thummi at larval and prepupal stages.

Authors: I I Kiknadze; I E Vlasova; A I Sherudilo
Journal: Cell Differ Date: 1975-03

3. [RIBONUCLEIC ACID SYNTHESIS IN GIANT CHROMOSOMES. AUTORADIOGRAPHIC INVESTIGATIONS ON CHIRONOMUS TENTANS].

Authors: C PELLING
Journal: Chromosoma Date: 1964-04-01 Impact factor: 4.316

4. [Induction of puff changes in the salivary gland chromosomes of Chironomus tentans by ecdysone].

Authors: U CLEVER; P KARLSON
Journal: Exp Cell Res Date: 1960-09 Impact factor: 3.905

5. [The puffs of salivary gland chromosomes of Drosophilia melanogaster. Part 1. Observations on the behavior of a typical puff in the normal strain and in two mutants, giant and lethal giant larvae].

Authors: H J BECKER
Journal: Chromosoma Date: 1959 Impact factor: 4.316

10. THE BASE COMPOSITION OF NUCLEIC ACIDS IN CHROMOSOMES, PUFFS, NUCLEOLI, AND CYTOPLASM OF CHIRONOMUS SALIVARY GLAND CELLS.

Authors: J E Edström; W Beermann
Journal: J Cell Biol Date: 1962-09-01 Impact factor: 10.539

1 in total

1. Chromatin structure of a hyperactive secretory protein gene (in Balbiani ring 2) of Chironomus.

Authors: R M Widmer; R Lucchini; M Lezzi; B Meyer; J M Sogo; J E Edström; T Koller
Journal: EMBO J Date: 1984-07 Impact factor: 11.598

1 in total

Isolation of DNA from single microsurgically excised bands of polytene chromosomes of Chironomus.

Review 1. Transcription in polytene chromosomes.

2. Quantitative analysis of DNA content in the salivary gland chromosomes of Chironomus thummi at larval and prepupal stages.

3. [RIBONUCLEIC ACID SYNTHESIS IN GIANT CHROMOSOMES. AUTORADIOGRAPHIC INVESTIGATIONS ON CHIRONOMUS TENTANS].

4. [Induction of puff changes in the salivary gland chromosomes of Chironomus tentans by ecdysone].

5. [The puffs of salivary gland chromosomes of Drosophilia melanogaster. Part 1. Observations on the behavior of a typical puff in the normal strain and in two mutants, giant and lethal giant larvae].

6. A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

7. RNA synthesis in puff 2-48BC after experimental induction in Drosophila hydei.

8. Cytophotometric and autoradiographic evidence for post-natal DNA synthesis in neurons of the rat cerebral cortex.

9. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.

10. THE BASE COMPOSITION OF NUCLEIC ACIDS IN CHROMOSOMES, PUFFS, NUCLEOLI, AND CYTOPLASM OF CHIRONOMUS SALIVARY GLAND CELLS.

1. Chromatin structure of a hyperactive secretory protein gene (in Balbiani ring 2) of Chironomus.