Literature DB >> 7069228

Purification and characterization of the mucinase of Vibrio cholerae.

D R Schneider, C D Parker.   

Abstract

Mucinase from Vibrio cholerae strain CA401 was purified by precipitation with ammonium sulfate and chromatography on a column of BioGel P-100 (Bio-Rad Laboratories, Richmond, California). Ovomucinase, intestinal mucinase, and protease appeared as two peaks of slightly different molecular weights, comigrated in nondenaturing polyacrylamide gel electrophoresis, demonstrated similar patterns of inhibition by heavy metals, and were inhibited by antiserum to purified mucinase. Both molecular weight forms exhibited similar properties, which were identical to those of fresh culture supernatants. Specific activity was only slightly increased by purification. Antiserum to mucinase passively protected infant mice from diarrhea due to V. cholerae.

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Year:  1982        PMID： 7069228     DOI： 10.1093/infdis/145.4.474

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  13 in total

1.  Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel.

Authors:  Martin E Lidell; Darcy M Moncada; Kris Chadee; Gunnar C Hansson
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-05       Impact factor: 11.205

2.  Rapid growth of planktonic Vibrio cholerae non-O1/non-O139 strains in a large alkaline lake in Austria: dependence on temperature and dissolved organic carbon quality.

Authors:  Alexander K T Kirschner; Jane Schlesinger; Andreas H Farnleitner; Romana Hornek; Beate Süss; Beate Golda; Alois Herzig; Bettina Reitner
Journal:  Appl Environ Microbiol       Date:  2008-02-01       Impact factor: 4.792

3.  Defective mucin-type glycosylation on α-dystroglycan in COG-deficient cells increases its susceptibility to bacterial proteases.

Authors:  Seok-Ho Yu; Peng Zhao; Pradeep K Prabhakar; Tiantian Sun; Aaron Beedle; Geert-Jan Boons; Kelley W Moremen; Lance Wells; Richard Steet
Journal:  J Biol Chem       Date:  2018-07-26       Impact factor: 5.157

4.  Production and partial characterization of an elastolytic protease of Vibrio vulnificus.

Authors:  M H Kothary; A S Kreger
Journal:  Infect Immun       Date:  1985-11       Impact factor: 3.441

5.  Vibrio cholerae hemagglutinin/lectin/protease hydrolyzes fibronectin and ovomucin: F.M. Burnet revisited.

Authors:  R A Finkelstein; M Boesman-Finkelstein; P Holt
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

6.  Vibrio cholerae soluble hemagglutinin/protease is a metalloenzyme.

Authors:  B A Booth; M Boesman-Finkelstein; R A Finkelstein
Journal:  Infect Immun       Date:  1983-11       Impact factor: 3.441

Review 7.  Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp.

Authors:  J M Janda; C Powers; R G Bryant; S L Abbott
Journal:  Clin Microbiol Rev       Date:  1988-07       Impact factor: 26.132

8.  Characterization of binding of Candida albicans to small intestinal mucin and its role in adherence to mucosal epithelial cells.

Authors:  L de Repentigny; F Aumont; K Bernard; P Belhumeur
Journal:  Infect Immun       Date:  2000-06       Impact factor: 3.441

9.  Successful colonization and immunization of adult rabbits by oral inoculation with Vibrio cholerae O1.

Authors:  W C Cray; E Tokunaga; N F Pierce
Journal:  Infect Immun       Date:  1983-08       Impact factor: 3.441

Review 10.  Effects of global climate on infectious disease: the cholera model.

Authors:  Erin K Lipp; Anwar Huq; Rita R Colwell
Journal:  Clin Microbiol Rev       Date:  2002-10       Impact factor: 26.132
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