Literature DB >> 7068757

Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction.

E J Patzer, D M Schlossman, J E Rothman.   

Abstract

Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.

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Year:  1982        PMID： 7068757      PMCID： PMC2112108          DOI： 10.1083/jcb.93.1.230

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  18 in total

1.  Coated vesicles from pig brain: purification and biochemical characterization.

Authors:  B M Pearse
Journal:  J Mol Biol       Date:  1975-09-05       Impact factor: 5.469

2.  Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts.

Authors:  R G Anderson; M S Brown; J L Goldstein
Journal:  Cell       Date:  1977-03       Impact factor: 41.582

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Coated vesicles: characterization, selective dissociation, and reassembly.

Authors:  M P Woodward; T F Roth
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

5.  Clathrin-coated vesicles: isolation, dissociation and factor-dependent reassociation of clathrin baskets.

Authors:  J H Keen; M C Willingham; I H Pastan
Journal:  Cell       Date:  1979-02       Impact factor: 41.582

6.  Mechanochemical properties of brain clathrin: interactions with actin and alpha-actinin and polymerization into basketlike structures or filaments.

Authors:  W Schook; S Puszkin; W Bloom; C Ores; S Kochwa
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

7.  Coated pits, coated vesicles, and receptor-mediated endocytosis.

Authors:  J L Goldstein; R G Anderson; M S Brown
Journal:  Nature       Date:  1979-06-21       Impact factor: 49.962

8.  Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction.

Authors:  J E Heuser; T S Reese
Journal:  J Cell Biol       Date:  1973-05       Impact factor: 10.539

9.  Evidence that coated vesicles isolated from brain are calcium-sequestering organelles resembling sarcoplasmic reticulum.

Authors:  A L Blitz; R E Fine; P A Toselli
Journal:  J Cell Biol       Date:  1977-10       Impact factor: 10.539

10.  Three-dimensional visualization of coated vesicle formation in fibroblasts.

Authors:  J Heuser
Journal:  J Cell Biol       Date:  1980-03       Impact factor: 10.539
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  13 in total

1.  Dissociation and reassembly of soybean clathrin.

Authors:  R E Wiedenhoeft; G W Schmidt; B A Palevitz
Journal:  Plant Physiol       Date:  1988-02       Impact factor: 8.340

2.  On the role of assembly kinetics in determining the structure of clathrin cages.

Authors:  B I Shraiman
Journal:  Biophys J       Date:  1997-02       Impact factor: 4.033

Review 3.  Receptor-mediated endocytosis.

Authors:  T Wileman; C Harding; P Stahl
Journal:  Biochem J       Date:  1985-11-15       Impact factor: 3.857

4.  Clathrin heavy chain, light chain interactions.

Authors:  F K Winkler; K K Stanley
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

5.  A monoclonal antibody to the heavy chain of clathrin.

Authors:  D Louvard; C Morris; G Warren; K Stanley; F Winkler; H Reggio
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

6.  Effects of cytoplasmic acidification on clathrin lattice morphology.

Authors:  J Heuser
Journal:  J Cell Biol       Date:  1989-02       Impact factor: 10.539

7.  Dissociation of clathrin coats coupled to the hydrolysis of ATP: role of an uncoating ATPase.

Authors:  W A Braell; D M Schlossman; S L Schmid; J E Rothman
Journal:  J Cell Biol       Date:  1984-08       Impact factor: 10.539

8.  Deep-etch visualization of proteins involved in clathrin assembly.

Authors:  J E Heuser; J Keen
Journal:  J Cell Biol       Date:  1988-09       Impact factor: 10.539

9.  An enzyme that removes clathrin coats: purification of an uncoating ATPase.

Authors:  D M Schlossman; S L Schmid; W A Braell; J E Rothman
Journal:  J Cell Biol       Date:  1984-08       Impact factor: 10.539

10.  Trimeric binding of the 70-kD uncoating ATPase to the vertices of clathrin triskelia: a candidate intermediate in the vesicle uncoating reaction.

Authors:  J Heuser; C J Steer
Journal:  J Cell Biol       Date:  1989-10       Impact factor: 10.539
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