Literature DB >> 7068433

Construction of human gene libraries from small amounts of peripheral blood: analysis of beta-like globin genes.

M Poncz, D Solowiejczyk, B Harpel, Y Mory, E Schwartz, S Surrey.   

Abstract

We describe a rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood. High molecular weight DNA is isolated from 5-20 ml peripheral blood, partially cleaved with Eco R1, and 8-22 kb fragments are cloned using bacteriophage Charon 4A and suitable E. coli host. Using the approach we have isolated and characterized several non-alpha globin clones from a Kurdish Jew with homozygous beta thalassemia. The ability to isolate suitable amounts of high molecular weight DNA from peripheral blood provides a relatively simple means of constructing human gene libraries representing a variety of hemoglobin disorders.

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Year:  1982        PMID： 7068433     DOI： 10.3109/03630268208996930

Source DB:  PubMed          Journal:  Hemoglobin        ISSN： 0363-0269            Impact factor:   0.849


  96 in total

1.  The molecular basis of beta-thalassemia in Turkey.

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2.  Selective expression of human X chromosome-linked green opsin genes.

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Journal:  Croat Med J       Date:  2006-06       Impact factor: 1.351

4.  Analysis of 5' flanking regions of the gamma globin genes from major African haplotype backgrounds associated with sickle cell disease.

Authors:  S R Month; R W Wood; P T Trifillis; P J Orchowski; B Sharon; S K Ballas; S Surrey; E Schwartz
Journal:  J Clin Invest       Date:  1990-02       Impact factor: 14.808

5.  The linkage of Hb Valletta [alpha 2 beta 287(f3)Thr----Pro] and Hb F-Malta-I [alpha 2G gamma 2117(G19)His----Arg] in the Maltese population.

Authors:  F Kutlar; A E Felice; J L Grech; W H Bannister; A Kutlar; J B Wilson; B B Webber; H Y Hu; T H Huisman
Journal:  Hum Genet       Date:  1991-04       Impact factor: 4.132

6.  Compound heterozygosity for a beta zero-thalassemia (frameshift codons 38/39; -C) and a nondeletional Swiss type of HPFH (A----C at NT -110, G gamma) in a Czechoslovakian family.

Authors:  K Indrak; J Indrakova; F Kutlar; D Pospisilova; I Sulovska; E Baysal; T H Huisman
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7.  Prevalence and Genetic Characterization of Glucose-6-Phosphate Dehydrogenase Deficiency in Anemic Subjects from Uttar Pradesh, India.

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Journal:  J Pediatr Genet       Date:  2019-01-30

8.  Cytogenetic and Y chromosome microdeletion screening studies in infertile males with Oligozoospermia and Azoospermia in Southeast Turkey.

Authors:  M Balkan; S Tekes; A Gedik
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Authors:  A Iolascon; T Parrella; S Perrotta; O Guardamagna; P M Coates; M Sartore; S Surrey; P Fortina
Journal:  J Med Genet       Date:  1994-07       Impact factor: 6.318

10.  Glutathione peroxidase 1 (GPX1) genetic polymorphism, erythrocyte GPX activity, and prostate cancer risk.

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Journal:  Int Urol Nephrol       Date:  2008-06-19       Impact factor: 2.370
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