Literature DB >> 7065905

A modified plaque assay method for accurate analysis of infectivity of influenza viruses with uncleaved hemagglutinin.

O P Zhirnov, A V Ovcharenko, A G Bukrinskaya.   

Abstract

A modified plaque assay method for evaluation of infectivity of influenza viruses is described. The modification consists of a double agar overlay, trypsin being included in the second layer added 24 hours after infection. The modified method allows one to determine accurately the infectivity titer of influenza viruses possessing uncleaved hemagglutinin, and to compare the infectivity of viruses possessing cleaved or uncleaved forms of hemagglutinin. The standard method in which trypsin is included into agar overlay just after infection artificially increases the infectivity titer of viruses with uncleaved hemagglutinin and therefore is not suitable for these purposes.

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Year:  1982        PMID： 7065905     DOI： 10.1007/bf01314887

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  16 in total

1.  Activation of influenza A viruses by trypsin treatment.

Authors:  H D Klenk; R Rott; M Orlich; J Blödorn
Journal:  Virology       Date:  1975-12       Impact factor: 3.616

2.  Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.

Authors:  A Scheid; P W Choppin
Journal:  Virology       Date:  1974-02       Impact factor: 3.616

3.  Trypsin action on the growth of Sendai virus in tissue culture cells. 3. Structural difference of Sendai viruses grown in eggs and tissue culture cells.

Authors:  M Homma; M Ouchi
Journal:  J Virol       Date:  1973-12       Impact factor: 5.103

4.  Plaque formation by influenza viruses in the presence of trypsin.

Authors:  G Appleyard; H B Maber
Journal:  J Gen Virol       Date:  1974-12       Impact factor: 3.891

5.  Separation of two polypeptide chains from the hemagglutinin subunit of influenza virus.

Authors:  W G Laver
Journal:  Virology       Date:  1971-07       Impact factor: 3.616

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  The function of the neuraminidase in membrane fusion induced by myxoviruses.

Authors:  R T Huang; R Rott; K Wahn; H D Klenk; T Kohama
Journal:  Virology       Date:  1980-12       Impact factor: 3.616

8.  Fusion between cell membrane and liposomes containing the glycoproteins of influenza virus.

Authors:  R T Huang; K Wahn; H D Klenk; R Rott
Journal:  Virology       Date:  1980-07-30       Impact factor: 3.616

9.  Genetic recombination for antigenic markers of antigenically different strains of influenza B virus.

Authors:  K Tobita; E D Kilbourne
Journal:  J Virol       Date:  1974-02       Impact factor: 5.103

10.  Influenza virus-specific T cell-mediated cytotoxicity: integration of the virus antigen into the target cell membrane is essential for target cell formation.

Authors:  R Kurrle; H Wagner; M Röllinghoff; R Rott
Journal:  Eur J Immunol       Date:  1979-02       Impact factor: 5.532
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  14 in total

1.  The host origin of influenza A viruses can be assessed by the intracellular cleavage of the viral nucleocapsid protein. Brief report.

Authors:  O P Zhirnov
Journal:  Arch Virol       Date:  1988       Impact factor: 2.574

2.  Cleavage of influenza A virus H1 hemagglutinin by swine respiratory bacterial proteases.

Authors:  R J Callan; F A Hartmann; S E West; V S Hinshaw
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

3.  Dynamics of biologically active subpopulations of influenza virus: plaque-forming, noninfectious cell-killing, and defective interfering particles.

Authors:  Philip I Marcus; John M Ngunjiri; Margaret J Sekellick
Journal:  J Virol       Date:  2009-06-03       Impact factor: 5.103

4.  Protective effect of protease inhibitors in influenza virus infected animals.

Authors:  O P Zhirnov; A V Ovcharenko; A G Bukrinskaya
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

5.  NS1 protein of influenza A virus down-regulates apoptosis.

Authors:  O P Zhirnov; T E Konakova; T Wolff; H-D Klenk
Journal:  J Virol       Date:  2002-02       Impact factor: 5.103

6.  Effects of bacterial microflora of the lower digestive tract of free-range waterfowl on influenza virus activation.

Authors:  Marcus D King; M Neal Guentzel; Bernard P Arulanandam; Adria A Bodour; Vinayak Brahmakshatriya; Blanca Lupiani; James P Chambers
Journal:  Appl Environ Microbiol       Date:  2011-04-29       Impact factor: 4.792

7.  Cleavage of influenza a virus hemagglutinin in human respiratory epithelium is cell associated and sensitive to exogenous antiproteases.

Authors:  Oleg P Zhirnov; Mine R Ikizler; Peter F Wright
Journal:  J Virol       Date:  2002-09       Impact factor: 5.103

8.  Pneumopathogenicity in mice of a Sendai virus mutant, TSrev-58, is accompanied by in vitro activation with trypsin.

Authors:  Y Mochizuki; M Tashiro; M Homma
Journal:  J Virol       Date:  1988-08       Impact factor: 5.103

9.  Replication of influenza B virus in chicken embryos is suppressed by exogenous aprotinin.

Authors:  O P Zhirnov; P B Golyando; A V Ovcharenko
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

10.  Clonogenic assay of type a influenza viruses reveals noninfectious cell-killing (apoptosis-inducing) particles.

Authors:  John M Ngunjiri; Margaret J Sekellick; Philip I Marcus
Journal:  J Virol       Date:  2008-01-09       Impact factor: 5.103
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