Literature DB >> 7065904

Affinity chromatography of viroid RNAs.

W Rohde.   

Abstract

The application of a structure- and GC-specific affinity adsorbent for the purification of viroid RNAs is described. Cross-linked bisacrylamide gels to which the intercalating dye phenyl neutral red is covalently attached [2] constitute a powerful tool for the rapid, quantitative, and selective isolation of viroid as well as viroid complementary RNAs from crude, plant polysaccharides containing nucleic acid fractions.

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Year:  1982        PMID： 7065904     DOI： 10.1007/bf01314886

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  9 in total

1.  Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.

Authors:  H L Sanger; G Klotz; D Riesner; H J Gross; A K Kleinschmidt
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  Properties of the complementary RNA sequences associated with infection by the citrus exocortis viroid.

Authors:  L K Grill; V I Negruk; J S Semancik
Journal:  Virology       Date:  1980-11       Impact factor: 3.616

3.  Base specific fractionation of double stranded DNA: affinity chromatography on a novel type of adsorbant.

Authors:  H Bünemann; W Müller
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

4.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

Review 5.  Viroids: a class of subviral pathogens.

Authors:  H J Gross; D Riesner
Journal:  Angew Chem Int Ed Engl       Date:  1980       Impact factor: 15.336

6.  Sequence-specific priming of the in vitro synthesis of DNA complementary to citrus exocortis viroid.

Authors:  W Rohde; M Schnölzer; H L Sänger
Journal:  FEBS Lett       Date:  1981-08-03       Impact factor: 4.124

7.  Structure and structure formation of viroids.

Authors:  D Riesner; K Henco; U Rokohl; G Klotz; A K Kleinschmidt; H Domdey; P Jank; H J Gross; H L Sänger
Journal:  J Mol Biol       Date:  1979-09-05       Impact factor: 5.469

8.  Detection of complementary RNA intermediates of viroid replication by Northern blot hybridization.

Authors:  W Rohde; H L Sänger
Journal:  Biosci Rep       Date:  1981-04       Impact factor: 3.840

9.  Specifically primed synthesis in vitro of full-length DNA complementary to potato-spindle-tuber viroid.

Authors:  W Rohde; M Schnölzer; H R Rackwitz; B Haas; H Seliger; H L Sänger
Journal:  Eur J Biochem       Date:  1981-08
  9 in total

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