Literature DB >> 7061865

An improved technique for the isolation of lymphocytes from small volumes of peripheral mouse blood.

F Mizobe, E Martial, S Colby-Germinario, B G Livett.   

Abstract

Centrifugation of mouse blood on density gradients of polyvinylpyrrolidone-coated colloidal silica (Percoll) resulted in the simultaneous separation of platelets, lymphocytes, granulocytes and erythrocytes. By this method, we have been successful in isolating a fraction of highly purified and viable lymphocytes from small volumes of peripheral mouse blood with good recovery.

Entities:  

Mesh:

Substances:

Year:  1982        PMID: 7061865     DOI: 10.1016/0022-1759(82)90327-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  1 in total

1.  Enhancement of in vitro prostaglandin E2 production by mouse fibrosarcoma cells after co-culture with various anti-tumour effector cells.

Authors:  F Okada; M Hosokawa; J Hasegawa; Y Kuramitsu; K Nakai; L Yuan; H Lao; H Kobayashi; N Takeichi
Journal:  Br J Cancer       Date:  1994-08       Impact factor: 7.640

  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.