Literature DB >> 7061832

A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.

D Pette, H Reichmann.   

Abstract

A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.

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Year:  1982        PMID： 7061832     DOI： 10.1177/30.4.7061832

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  15 in total

1.  Metabolic patterns in meningiomas.

Authors:  Birgit Herting; Jürgen Meixensberger; Wolfgang Roggendorf; Heinz Reichmann
Journal:  J Neurooncol       Date:  2003-11       Impact factor: 4.130

2.  Fibre types, calcium-sequestering proteins and metabolic enzymes in denervated and chronically stimulated muscles of the rat.

Authors:  K Gundersen; E Leberer; T Lømo; D Pette; R S Staron
Journal:  J Physiol       Date:  1988-04       Impact factor: 5.182

3.  Fibre type specific transformations in the enzyme activity pattern of rat vastus lateralis muscle by prolonged endurance training.

Authors:  H J Green; H Reichmann; D Pette
Journal:  Pflugers Arch       Date:  1983-11       Impact factor: 3.657

4.  Relationships between early alterations in parvalbumins, sarcoplasmic reticulum and metabolic enzymes in chronically stimulated fast twitch muscle.

Authors:  G Klug; W Wiehrer; H Reichmann; E Leberer; D Pette
Journal:  Pflugers Arch       Date:  1983-12       Impact factor: 3.657

5.  Effects of chronic stimulation on the metabolic heterogeneity of the fibre population in rabbit tibialis anterior muscle.

Authors:  A Buchegger; P M Nemeth; D Pette; H Reichmann
Journal:  J Physiol       Date:  1984-05       Impact factor: 5.182

6.  Ipsi- and contralateral fibre transformations by cross-reinnervation. A principle of symmetry.

Authors:  H Reichmann; T Srihari; D Pette
Journal:  Pflugers Arch       Date:  1983-05       Impact factor: 3.657

7.  Analyses of energy metabolism and mitochondrial genome in post-mortem brain from patients with Alzheimer's disease.

Authors:  H Reichmann; S Flörke; G Hebenstreit; H Schrubar; P Riederer
Journal:  J Neurol       Date:  1993-06       Impact factor: 4.849

8.  Species-specific effects of chronic nerve stimulation upon tibialis anterior muscle in mouse, rat, guinea pig, and rabbit.

Authors:  J A Simoneau; D Pette
Journal:  Pflugers Arch       Date:  1988-07       Impact factor: 3.657

9.  Neural regulation of parvalbumin expression in mammalian skeletal muscle.

Authors:  E Leberer; D Pette
Journal:  Biochem J       Date:  1986-04-01       Impact factor: 3.857

10.  Neural control of gene expression in skeletal muscle. Calcium-sequestering proteins in developing and chronically stimulated rabbit skeletal muscles.

Authors:  E Leberer; U Seedorf; D Pette
Journal:  Biochem J       Date:  1986-10-15       Impact factor: 3.857
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