Literature DB >> 7061386

Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.

G Cukor, N A Nowak.   

Abstract

The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NAD-agarose resins were not efficient substrates for toxin purification.

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Year:  1982        PMID： 7061386      PMCID： PMC216513          DOI： 10.1128/jb.149.3.1162-1165.1982

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.476


  10 in total

1.  Toxin inhibitors of protein synthesis: production, purification, and assay of Pseudomonas aeruginosa toxin A.

Authors:  B H Iglewski; J C Sadoff
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  Affinity chromatography purification of diphtheria toxin.

Authors:  G Cukor; J D Readio; R J Kuchler
Journal:  Biotechnol Bioeng       Date:  1974-07       Impact factor: 4.530

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A.

Authors:  P V Liu
Journal:  J Infect Dis       Date:  1973-10       Impact factor: 5.226

5.  Chimeric toxins: toxic, disulfide-linked conjugate of concanavalin A with fragment A from diphtheria toxin.

Authors:  D G Gilliland; R J Collier; J M Moehring; T J Moehring
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

6.  Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa.

Authors:  D W Chung; R J Collier
Journal:  Infect Immun       Date:  1977-06       Impact factor: 3.441

7.  Large-scale purification and characterization of the exotoxin of Pseudomonas aeruginosa.

Authors:  S H Leppla
Journal:  Infect Immun       Date:  1976-10       Impact factor: 3.441

8.  General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.

Authors:  K Mosbach; H Guilford; R Ohlsson; M Scott
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857

9.  Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa.

Authors:  D E Ohman; R P Burns; B H Iglewski
Journal:  J Infect Dis       Date:  1980-10       Impact factor: 5.226

10.  Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum.

Authors:  L T Callahan
Journal:  Infect Immun       Date:  1976-07       Impact factor: 3.441
  10 in total
  2 in total

Review 1.  Global discovery of protein kinases and other nucleotide-binding proteins by mass spectrometry.

Authors:  Yongsheng Xiao; Yinsheng Wang
Journal:  Mass Spectrom Rev       Date:  2014-11-05       Impact factor: 10.946

2.  Comparative analysis of serum antibody responses to Pseudomonas aeruginosa exotoxin A by cystic fibrosis and intensive care unit patients.

Authors:  G Cukor; N R Blacklow; N A Nowak; C M Rich; L E Braverman; R A Fischer
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 11.677
  2 in total

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