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Literature DB >> 7061381

Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.

M H Shirvan, S Rottem, Z Ne'eman, R Bittman.

Abstract

A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity and interferes with the regulation of cell volume. The procedure can be used to isolate membranes of other osmotically resistant mycoplasmas.

Entities: Chemical Disease Species

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Year: 1982 PMID： 7061381 PMCID： PMC216504 DOI： 10.1128/jb.149.3.1124-1128.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

13 in total

1. Membrane lipids of Mycoplasma gallisepticum: a disaturated phosphatidylcholine and a phosphatidylglycerol with an unusual positional distribution of fatty acids.

Authors: S Rottem; O Markowitz
Journal: Biochemistry Date: 1979-07-10 Impact factor: 3.162

2. Fractionation of mycoplasma cells for enzyme localization.

Authors: J D Pollack; S Razin; M E Pollack; R C Cleverdon
Journal: Life Sci Date: 1965-05 Impact factor: 5.037

3. Reassembly of Mycoplasma membranes disaggregated by detergents.

Authors: S Rottem; O Stein; S Razin
Journal: Arch Biochem Biophys Date: 1968-04 Impact factor: 4.013

4. Proton motive force across the membrane of Mycoplasma gallisepticum and its possible role in cell volume regulation.

Authors: S Rottem; C Linker; T H Wilson
Journal: J Bacteriol Date: 1981-03 Impact factor: 3.490

5. The antigens of Mycoplasma hominis.

Authors: M R Hollingdale; R M Lemcke
Journal: J Hyg (Lond) Date: 1969-12

6. Changes in composition, biosynthesis, and physical state of membrane lipids occurring upon aging of Mycoplasma hominis cultures.

Authors: S Rottem; A S Greenberg
Journal: J Bacteriol Date: 1975-02 Impact factor: 3.490

7. Isolation of mycoplasma membranes by digitonin.

Authors: S Rottem; S Razin
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

8. Isolation, chemical composition, and ultrastructural features of the cell membrane of the mycoplasma-like organism Spiroplasma citri.

Authors: S Razin; M Hasin; Z Ne'eman; S Rottem
Journal: J Bacteriol Date: 1973-12 Impact factor: 3.490

9. Electrophoretic analysis of the arrangement of spiralin and other major proteins in isolated Spiroplasma citri cell membranes.

Authors: H Wróblewski
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490