The biosynthesis and linkage of teichuronic acid to peptidoglycan in Bacillus licheniformis.

Membrane and wall-membrane preparations of Bacillus licheniformis 94 will, if supplied with the appropriate precursors, synthesize teichuronic acid and link it to peptidoglycan although teichuronic acid is absent from walls of this organism. B. licheniformis 94 lacks phosphoglucomutase activity and therefore cannot synthesize the precursor UDPglucuronic acid. The initial reaction of teichuronic acid biosynthesis is catalysed by a translocase and results in the formation of polyprenyl-diphospho-N-acetylgalactosamine and the release of UMP. This reaction is not inhibited by tunicamycin. The disaccharide repeating unit of the polymer is then formed by the transfer of glucuronic acid from UDPglucuronic acid with the release of UDP. Polymerization of the repeating units occurs by incorporation of new units at the reducing terminus of the growing teichuronic acid chain and the release of polyprenyl diphosphate. The subsequent dephosphorylation of the lipid diphosphate for reuse in the biosynthesis cycle is inhibited by bacitracin. Linkage to peptidoglycan occurs by the formation of a phosphodiester bond between the reducing N-acetylgalactosamine terminus of the teichuronic acid chain and a 6-hydroxyl group of a muramic acid residue in the glycan of peptidoglycan. Wall-membrane preparations synthesizing teichuronic acid, poly(glycerol phosphate) teichoic acid and peptidoglycan link the teichuronic and teichoic acids to different glycan chains.