Literature DB >> 7059603

A rapid and sensitive assay for determination of cholesterol in membrane lipid extracts.

P Ott, Y Binggeli, U Brodbeck.   

Abstract

A commercially available enzymatic assay (Boehringer Monotest) was modified to allow a rapid and sensitive determination of cholesterol in membrane lipid extracts. This was achieved by adding 0.5% Triton X-100 to the reagent solution. The detergent did not interfere with the assay. The relationship between the amount of cholesterol per assay and the absorbance at 500 nm was linear up to 100 micrograms. The recovery in the assay was better than 95%. The assay was applied to the determination of cholesterol in erythrocyte membrane lipid extracts.

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Year:  1982        PMID： 7059603     DOI： 10.1016/0005-2736(82)90101-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  10 in total

1.  Effect of pentoxifylline on erythrocyte membrane and plasma lipids.

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2.  Fish diet and physical fitness in relation to membrane and serum lipids, prostanoid metabolism and platelet aggregation in female students.

Authors:  J J Agren; H Pekkarinen; H Litmanen; O Hänninen
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3.  Mevinolin, an inhibitor of cholesterol biosynthesis, drastically depresses Ca2+ channel activity and uncouples excitation from contraction in cardiac cells in culture.

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4.  Lipid peroxidation and physical properties of smooth and rough hepatoma microsomes.

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Journal:  Cell Biophys       Date:  1989-02

5.  The effects of atorvastatin therapy on rheological characteristics of erythrocyte membrane, serum lipid profile and oxidative status in patients with dyslipidemia.

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6.  Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia.

Authors:  H D Luján; M R Mowatt; L G Byrd; T E Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

7.  Normal serum and lipoprotein-deficient serum give different expressions of excitability, corresponding to different stages of differentiation, in chicken cardiac cells in culture.

Authors:  J F Renaud; A M Scanu; T Kazazoglou; A Lombet; G Romey; M Lazdunski
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

8.  Organization and dynamics of lipids in bovine brain coated and uncoated vesicles.

Authors:  A Alfsen; C de Paillerets; K Prasad; P K Nandi; R E Lippoldt; H Edelhoch
Journal:  Eur Biophys J       Date:  1984       Impact factor: 1.733

9.  Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol.

Authors:  Ekambaram Padmini; Balasubramaniam Thiripura Sundari
Journal:  J Clin Biochem Nutr       Date:  2008-01       Impact factor: 3.114

10.  Cholesterol forms and traditional lipid profile for projection of atherogenic dyslipidemia: lipoprotein subfractions and erythrocyte membrane cholesterol.

Authors:  Hüseyin Avni Uydu; Mehmet Bostan; Mehtap Atak; Adnan Yılmaz; Adem Demir; Buket Akçan; Fatih Sümer; Nimet Baltaş; Zakir Karadağ; Yavuz Uğurlu; Asım Orem
Journal:  J Membr Biol       Date:  2013-11-16       Impact factor: 1.843
  10 in total

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