Determination with Hi-Gonavis of luteinizing hormone levels in urine compared with those in plasma.

Hi-Gonavis, an immunological luteinizing hormone/human chorionic gonadotrophin (LH/hCG) test kit, was used to measure the periovulatory LH excretion in the urine of 25 patients over two cycles. The LH plasma levels was simultaneously determined by radioimmunoassay. The lower detection limit of Hi-Gonavis is 12.5 i.u. of LH/l of urine. Urinary LH levels of 50 i.u./l were associated with plasma levels of at least 12 i.u./l. Such values were found usually on the day before the mid-cycle peak of LH. The pre-ovulatory increasing plasma LH levels correlated closely with the urinary values. The urinary LH level reflects that in plasma; the Hi-Gonavis method can be used to predict the occurrence of ovulation.