Separation of mononuclear and polymorphonuclear leucocytes from human blood by the one-step Hypaque-Ficoll method is dependent on blood column height.

Further observations have been made in relation to separation of mononuclear (MN) and polymorphonuclear (PMN) leucocytes from human blood by the one-step procedure involving centrifugation of the blood on Hypaque-Ficoll medium. The distance separating the MN and PMN cell fractions was dependent on the height of the blood column but independent of the diameter of the tube, this distance being directly proportional to the height of blood. However, increasing the height of the blood column by diluting the blood in medium 199 did not increase the separation distance. Erythrocytes were shown to be important for separation of leucocytes into two distinct bands.