Literature DB >> 7054274

Aluminum phosphate visualisation of acid phosphatase activity: a biochemical and x-ray microanalysis study.

J P Berry, J Hourdry, M Sternberg, P Galle.   

Abstract

A new method is described that demonstrates acid phosphatase activity in the cells of the proximal tubules of the rat kidney. The method is based on the formation of an insoluble aluminum phosphate precipitate. Microanalysis was used to demonstrate the presence of intracellular aluminum and determine the quantity present under the probe. Parallel biochemical studies showed that the aluminum precipitate was indeed due to acid phosphatase activity.

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Year:  1982        PMID: 7054274     DOI: 10.1177/30.1.7054274

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN: 0022-1554            Impact factor:   2.479


  7 in total

1.  Presence of lysosomal enzymes in the normal glomerular basement membrane matrix.

Authors:  A K Singh
Journal:  Histochem J       Date:  1993-08

Review 2.  X-ray microanalysis: a histochemical tool for elemental analysis.

Authors:  A T Sumner
Journal:  Histochem J       Date:  1983-06

3.  Use of laser microprobe mass analysis (LAMMA) for localizing multiple elements in soft and hard tissues.

Authors:  A H Verbueken; F L Van de Vyver; W J Visser; F Roels; R E Van Grieken; M E De Broe
Journal:  Biol Trace Elem Res       Date:  1987-08       Impact factor: 3.738

4.  Selective intra-lysosomal concentration of niobium in kidney and bone marrow cells: a microanalytical study.

Authors:  J P Berry; F Bertrand; P Galle
Journal:  Biometals       Date:  1993       Impact factor: 2.949

5.  Heavy metals in the terrestrial isopod Porcellio scaber Latreille. II. Subcellular fractionation of metal-accumulating lysosomes from hepatopancreas.

Authors:  R Dallinger; F Prosi
Journal:  Cell Biol Toxicol       Date:  1988-03       Impact factor: 6.691

6.  Role of alveolar macrophages in precipitation of mineral elements inhaled as soluble aerosols.

Authors:  P Galle; J P Berry; C Galle
Journal:  Environ Health Perspect       Date:  1992-07       Impact factor: 9.031

7.  Characterization by scanning transmission electron microscopy of silica particles from alveolar macrophages of coal miners.

Authors:  L C Rainey; P Bolsaitis; B Dirsa; J B Vander Sande
Journal:  Environ Health Perspect       Date:  1994-10       Impact factor: 9.031

  7 in total

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