Literature DB >> 7052942

Colorimetric estimation of phospholipids in aqueous dispersions.

R M Hallen.   

Abstract

A method for the estimation of phospholipids in aqueous dispersions is described. The method is based on the formation of a lipid-molybdenum blue complex, which is extracted into chloroform from the aqueous phase. Phosphate ions, detergents, proteins, neutral lipids and various other ions do not interfere in the lipid estimation. The method is sensitive down to a lipid concentration of 0.1 mumol/ml, with an accuracy better than +/- 3%.

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Year:  1980        PMID: 7052942     DOI: 10.1016/0165-022x(80)90049-4

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  3 in total

1.  Interaction of cationic colloids at the surface of J774 cells: a kinetic analysis.

Authors:  P Chenevier; B Veyret; D Roux; N Henry-Toulmé
Journal:  Biophys J       Date:  2000-09       Impact factor: 4.033

2.  Transport by reconstituted lactose carrier from parental and mutant strains of Escherichia coli.

Authors:  D Seto-Young; S Bedu; T H Wilson
Journal:  J Membr Biol       Date:  1984       Impact factor: 1.843

3.  Effect of different phospholipids on the reconstitution of two functions of the lactose carrier of Escherichia coli.

Authors:  D Seto-Young; C C Chen; T H Wilson
Journal:  J Membr Biol       Date:  1985       Impact factor: 1.843

  3 in total

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