Literature DB >> 7051451

Metacyclogenesis of Trypanosoma cruzi in vitro: a simplified procedure.

J J Sullivan.   

Abstract

A simplified procedure for obtaining high levels of infective, metacyclic trypomastigotes of a Brazilian strain of Trypanosoma cruzi in vitro is described. Epimastigotes cultured in Yaeger's Liver Infusion Tryptose Medium, when transferred to Grace's Insect Tissue Culture Medium (GMA) supplemented with 10% foetal bovine serum, yielded over 90% metacyclic forms. Decreasing or eliminating the serum from GMA adversely affected growth but did not decrease levels of transformation, thus providing a chemically defined culture system for analysing factors influencing metacyclogenesis of T. cruzi.

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Year:  1982        PMID： 7051451     DOI： 10.1016/0035-9203(82)90173-0

Source DB:  PubMed          Journal:  Trans R Soc Trop Med Hyg        ISSN： 0035-9203            Impact factor:   2.184


  9 in total

1.  Purification of metacyclic forms of Trypanosoma cruzi by Percoll discontinuous gradient centrifugation.

Authors:  S Castanys; A Osuna; F Gamarro; L M Ruiz-Pérez
Journal:  Z Parasitenkd       Date:  1984

2.  Gastric invasion by Trypanosoma cruzi and induction of protective mucosal immune responses.

Authors:  D F Hoft; P L Farrar; K Kratz-Owens; D Shaffer
Journal:  Infect Immun       Date:  1996-09       Impact factor: 3.441

3.  Purification of a Trypanosoma cruzi membrane glycoprotein which elicits lytic antibodies.

Authors:  K A Norris; G Harth; M So
Journal:  Infect Immun       Date:  1989-08       Impact factor: 3.441

4.  Purification of metacyclic trypomastigotes of Trypanosoma cruzi and Trypanosoma dionisii from culture using an epimastigote-specific monoclonal antibody.

Authors:  K Petry; J Schottelius; T Baltz
Journal:  Parasitol Res       Date:  1987       Impact factor: 2.289

5.  Evasion of alternative complement pathway by Trypanosoma cruzi results from inefficient binding of factor B.

Authors:  K Joiner; A Sher; T Gaither; C Hammer
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

6.  The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements.

Authors:  Eva Gluenz; Martin C Taylor; John M Kelly
Journal:  Int J Parasitol       Date:  2006-12-29       Impact factor: 3.981

7.  gp72, the 72 kilodalton glycoprotein, is the membrane acceptor site for C3 on Trypanosoma cruzi epimastigotes.

Authors:  K Joiner; S Hieny; L V Kirchhoff; A Sher
Journal:  J Exp Med       Date:  1985-05-01       Impact factor: 14.307

8.  A Novel Method for Inducing Amastigote-To-Trypomastigote Transformation In Vitro in Trypanosoma cruzi Reveals the Importance of Inositol 1,4,5-Trisphosphate Receptor.

Authors:  Muneaki Hashimoto; Jorge Morales; Haruki Uemura; Katsuhiko Mikoshiba; Takeshi Nara
Journal:  PLoS One       Date:  2015-08-12       Impact factor: 3.240

9.  In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium.

Authors:  Jessica Rodríguez Durán; Arturo Muñoz-Calderón; Karina Andrea Gómez; Mariana Potenza
Journal:  STAR Protoc       Date:  2021-09-01
  9 in total

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