Studies on ADCC assay using antigen-coated cells as target for detection of anti-LP-1 and anti-Tamm-Horsfall glycoprotein.

To detect anti-LP-1 and anti-Tamm-Horsfall glycoprotein (THGP), ADCC assay was attempted and in this assay antigen-coated FL cells were used as target. Dilution of rabbit antisera resulted in decreased % cytotoxicity but higher serum concentration than 10(4) x dilution of anti-LP-1 gave reduced % cytotoxicity. By absorption of anti-LP-1 with LP-1, the cytotoxicity was reduced, and by absorption of anti-THGP with LP-1 or THGP, the cytotoxicity was decreased in the same manner, suggesting that THGP might be existed in LP-1 preparation. The cytotoxicity was reduced when non-IgG-sheep red blood cell rosette forming cells were used as effector cells. So, it was suggested that in this assay the antibody specific to the corresponding antigen was required for the cytotoxicity and that lymphocytes bearing Fc-receptors might play a major role in the cytotoxicity of this assay. Conclusively this ADCC assay could be useful for detection of anti-LP-1 and anti-THGP in patients' sera.