Literature DB >> 7046811

An immunoradiometric quantitative assay of Escherichia coli recA protein.

C Paoletti, B Salles, P Giacomoni.   

Abstract

A two-site immunoradiometric assay of Escherichia coli recA protein is described ; its sensitivity allows the detection of 0.1 ng of recA protein ; it yields a linear response for amounts of recA protein in the 0.1-7 ng range. It can be directly applied to extracts of Escherichia coli obtained by sonication. Salmonella typhimurium extracts contain some cross-reacting material which share common antigenic determinants with Escherichia coli recA protein but differ from it.

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Year:  1982        PMID： 7046811     DOI： 10.1016/s0300-9084(82)80490-2

Source DB:  PubMed          Journal:  Biochimie        ISSN： 0300-9084            Impact factor:   4.079


  10 in total

1.  Immunological quantification of RecA protein in cell extracts of E. coli after exposure to chemical mutagens or UV radiation.

Authors:  I Fridrichová; A Kovarík; O Rosskopfová
Journal:  Folia Microbiol (Praha)       Date:  1992       Impact factor: 2.099

2.  Modulation of the SOS response by truncated RecA proteins.

Authors:  F Larminat; M Defais
Journal:  Mol Gen Genet       Date:  1989-03

3.  Weigle reactivation and mutagenesis of bacteriophage lambda in lexA(Def) mutants of E. coli K12.

Authors:  P Calsou; M Defais
Journal:  Mol Gen Genet       Date:  1985

4.  Activated RecA protein may induce expression of a gene that is not controlled by the LexA repressor and whose function is required for mutagenesis and repair of UV-irradiated bacteriophage lambda.

Authors:  P Calsou; A Villaverde; M Defais
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

5.  Measurement of in vivo expression of the recA gene of Escherichia coli by using lacZ gene fusions.

Authors:  J M Weisemann; C Funk; G M Weinstock
Journal:  J Bacteriol       Date:  1984-10       Impact factor: 3.490

6.  Regulation of the SOS response analyzed by RecA protein amplification.

Authors:  P Calsou; B Salles
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

7.  Control of UV induction of recA protein.

Authors:  B Salles; C Paoletti
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

8.  Lack of single-strand DNA-binding protein amplification under conditions of SOS induction in E. coli.

Authors:  B Salles; C Paoletti; G Villani
Journal:  Mol Gen Genet       Date:  1983

9.  Different levels of induction of RecA protein in E. coli (PQ 10) after treatment with two related carcinogens.

Authors:  B Salles; M C Lang; A M Freund; C Paoletti; M Daune; R P Fuchs
Journal:  Nucleic Acids Res       Date:  1983-08-11       Impact factor: 16.971

10.  Cell survival, UV-reactivation and induction of prophage lambda in Escherichia coli K12 overproducing RecA protein.

Authors:  P Quillardet; P L Moreau; H Ginsburg; D W Mount; R Devoret
Journal:  Mol Gen Genet       Date:  1982
  10 in total

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