Literature DB >> 7045487

High cloning efficiency of human lymphoid cells in agarose without feeder layers.

K N Ayres.   

Abstract

A protocol has been developed that consistently gives high cloning efficiencies for human lymphoid cell lines (lymphoblastoid and lymphoma) in agarose without the use of feeder layers. This procedure utilizes a cloning medium that contains horse serum, alpha-ketoglutarate or oxalacetate, and high levels of glutamine.

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Year:  1982        PMID： 7045487

Source DB:  PubMed          Journal:  J Natl Cancer Inst        ISSN： 0027-8874            Impact factor:   13.506


  3 in total

1.  Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridization.

Authors:  K Ayres; R Sklar; K Larson; V Lindgren; B Strauss
Journal:  Mol Cell Biol       Date:  1982-08       Impact factor: 4.272

2.  Hormone supplemented media for cloning human breast cancer: increased colony formation without alteration of chemosensitivity.

Authors:  F Calvo; D N Carney; M Brower; J D Minna
Journal:  Br J Cancer       Date:  1983-11       Impact factor: 7.640

3.  Heterogeneous origin of established non-T,non-B cell lines from two adolescent patients with acute lymphoblastic leukemia.

Authors:  M Nagasaka-Yabe; S Maeda; O Mabuchi; H Misu; S Nakamura; Y Kobayashi; H Hirai; T Sugiyama
Journal:  Jpn J Cancer Res       Date:  1988-01
  3 in total

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