Literature DB >> 7045233

A one-step procedure for preparation of classical pathway (C1q) and alternative pathway (factor D) depleted human serum.

F Praz, M C Roque Antunes Barreira, P Lesavre.   

Abstract

A simple method for preparation of a serum depleted in both C1q and Factor D is described. The hemolytic activities of both pathways are completely abolished and can be fully restored using the respective purified complement components. Furthermore, this serum is useful for studying cell systems since blocking either complement pathway does not require chelating agents.

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Year:  1982        PMID: 7045233     DOI: 10.1016/0022-1759(82)90229-0

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  10 in total

1.  Complement activation by apoptotic endothelial cells following hypoxia/reoxygenation.

Authors:  C Mold; C A Morris
Journal:  Immunology       Date:  2001-03       Impact factor: 7.397

2.  Calcium-independent haemolysis via the lectin pathway of complement activation in the guinea-pig and other species*.

Authors:  Y Zhang; C Suankratay; X Zhang; D R Jones; T F Lint; H Gewurz
Journal:  Immunology       Date:  1999-08       Impact factor: 7.397

3.  Formation of soluble immune complexes by complement in sera of patients with various hypocomplementemic states. Difference between inhibition of immune precipitation and solubilization.

Authors:  J A Schifferli; G Steiger; G Hauptmann; P J Spaeth; A G Sjöholm
Journal:  J Clin Invest       Date:  1985-12       Impact factor: 14.808

4.  Fast liver catabolism of C1q in patients with paraproteinaemia and depletion of the classical pathway of complement.

Authors:  J A Schifferli; M Pascual; G Steiger; M Schapira; J E Ryser; J Estreicher; A Dash
Journal:  Clin Exp Immunol       Date:  1987-07       Impact factor: 4.330

5.  Analysis of complement C3 deposition and degradation on Klebsiella pneumoniae.

Authors:  S Albertí; D Alvarez; S Merino; M T Casado; F Vivanco; J M Tomás; V J Benedí
Journal:  Infect Immun       Date:  1996-11       Impact factor: 3.441

6.  A family with complement factor D deficiency.

Authors:  D H Biesma; A J Hannema; H van Velzen-Blad; L Mulder; R van Zwieten; I Kluijt; D Roos
Journal:  J Clin Invest       Date:  2001-07       Impact factor: 14.808

7.  Human mannose-binding protein activates the alternative complement pathway and enhances serum bactericidal activity on a mannose-rich isolate of Salmonella.

Authors:  J E Schweinle; R A Ezekowitz; A J Tenner; M Kuhlman; K A Joiner
Journal:  J Clin Invest       Date:  1989-12       Impact factor: 14.808

8.  Mechanism of complement-dependent haemolysis via the lectin pathway: role of the complement regulatory proteins.

Authors:  C Suankratay; C Mold; Y Zhang; T F Lint; H Gewurz
Journal:  Clin Exp Immunol       Date:  1999-09       Impact factor: 4.330

9.  Activation of the alternative and classical complement pathways by Entamoeba histolytica.

Authors:  J Calderon; R D Schreiber
Journal:  Infect Immun       Date:  1985-11       Impact factor: 3.441

10.  Complement classical pathway components are all important in clearance of apoptotic and secondary necrotic cells.

Authors:  B Gullstrand; U Mårtensson; G Sturfelt; A A Bengtsson; L Truedsson
Journal:  Clin Exp Immunol       Date:  2009-03-02       Impact factor: 4.330

  10 in total

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