Effect of growth medium on the lipid composition of log and stationary phase cultures of Salmonella typhimurium.

The lipid content of Salmonella typhimurium was not significantly influenced by the carbon source present in the growth medium. The principal fatty acid was palmitic acid. Significant levels of palmitoleic acid were found in log phase cultures, and this was replaced by a C17 delta fatty acid in the stationary phase. The other fatty acids present in significant quantities were lauric, myristic, cis-vaccenic and linoleic acids. The phospholipids present were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidic acid and phosphatidylserine.