Literature DB >> 7041257

Expression of Treponema pallidum antigens in Escherichia coli.

A M Walfield, P A Hanff, M A Lovett.   

Abstract

Treponema pallidum DNA was cloned in a bacteriophage. Clones were screened for expression of Treponema pallidum antigens by an in situ radioimmunoassay on nitrocellulose, with the use of subsequent reactions with syphilitic serum and radioiodinated Staphylococcus aureus protein A. One clone, which gave a strong signal, codes for at least seven antigens that react specifically with human antibodies to Treponema pallidum.

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Year:  1982        PMID： 7041257     DOI： 10.1126/science.7041257

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  31 in total

1.  Primary structure of an oligomeric antigen of Treponema pallidum.

Authors:  A M Walfield; E S Roche; M C Zounes; H Kirkpatrick; M A Wild; G Textor; P K Tsai; C Richardson
Journal:  Infect Immun       Date:  1989-02       Impact factor: 3.441

2.  Molecular cloning and characterization of DNA from human intestinal spirochetes.

Authors:  G Dettori; R Burioni; R Grillo; P Cattani
Journal:  Eur J Epidemiol       Date:  1992-03       Impact factor: 8.082

Review 3.  The Treponema pallidum Outer Membrane.

Authors:  Justin D Radolf; Sanjiv Kumar
Journal:  Curr Top Microbiol Immunol       Date:  2018       Impact factor: 4.291

4.  Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star).

Authors:  K M Peterson; J B Baseman; J F Alderete
Journal:  Genitourin Med       Date:  1987-10

5.  Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen.

Authors:  T E Fehniger; A M Walfield; T M Cunningham; J D Radolf; J N Miller; M A Lovett
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

6.  Molecular cloning of the major outer membrane protein of Chlamydia trachomatis.

Authors:  I Allan; T M Cunningham; M A Lovett
Journal:  Infect Immun       Date:  1984-09       Impact factor: 3.441

7.  Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis.

Authors:  L M Schouls; O E Ijsselmuiden; J Weel; J D van Embden
Journal:  Infect Immun       Date:  1989-09       Impact factor: 3.441

8.  Serological characterization and gene localization of an Escherichia coli-expressed 37-kilodalton Treponema pallidum antigen.

Authors:  G C Rodgers; W J Laird; S R Coates; D H Mack; M Huston; J J Sninsky
Journal:  Infect Immun       Date:  1986-07       Impact factor: 3.441

9.  Identification and purification of a recombinant Treponema pallidum basic membrane protein antigen expressed in Escherichia coli.

Authors:  W S Dallas; P H Ray; J Leong; C D Benedict; L V Stamm; P J Bassford
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

10.  Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli.

Authors:  J E Thole; H G Dauwerse; P K Das; D G Groothuis; L M Schouls; J D van Embden
Journal:  Infect Immun       Date:  1985-12       Impact factor: 3.441
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