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Literature DB >> 7040393

Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli.

T Tsuchiya, K Ottina, Y Moriyama, M J Newman, T H Wilson.

Abstract

A strain of EScherichia coli was constructed containing a plasmid from the Clarke-Carbon collection that showed high levels of melibiose transport activity. Membranes from the plasmid-containing strain were extracted with octyl-beta-D-glucopyranoside and melibiose transport was reconstituted in liposomes. The proteoliposomes exhibited counterflow activity, as well as membrane potential and sodium gradient-driven melibiose accumulation.

Entities: Chemical Species

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Year: 1982 PMID： 7040393

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

11 in total

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Authors: Jason A Hall; Ana M Pajor
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2. Cloning of the tyrP gene and further characterization of the tyrosine-specific transport system in Escherichia coli K-12.

Authors: P J Wookey; J Pittard; S M Forrest; B E Davidson
Journal: J Bacteriol Date: 1984-10 Impact factor: 3.490

3. Antitermination of transcription from an Escherichia coli ribosomal RNA promoter.

Authors: W E Holben; E A Morgan
Journal: Proc Natl Acad Sci U S A Date: 1984-11 Impact factor: 11.205

Review 4. Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl/KCl symport system in animal cells.

Authors: M H Saier; D A Boyden
Journal: Mol Cell Biochem Date: 1984 Impact factor: 3.396

Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli.

1. Functional reconstitution of SdcS, a Na+-coupled dicarboxylate carrier protein from Staphylococcus aureus.

2. Cloning of the tyrP gene and further characterization of the tyrosine-specific transport system in Escherichia coli K-12.

3. Antitermination of transcription from an Escherichia coli ribosomal RNA promoter.

Review 4. Mechanism, regulation and physiological significance of the loop diuretic-sensitive NaCl/KCl symport system in animal cells.

Review 5. Gene-protein index of Escherichia coli K-12.

6. Partial characterization of a lysU mutant of Escherichia coli K-12.

Review 7. Proton-linked sugar transport systems in bacteria.

8. Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry.

9. Cloning in Escherichia coli K-12 of a Na+-dependent transport system from a marine bacterium.

10. Isolation of carbohydrate-reactive outer membrane proteins of Aeromonas hydrophila.