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Literature DB >> 7038680

traG protein of the F sex factor of Escherichia coli K-12 and its role in conjugation.

Abstract

The traG protein of the F sex factor is an inner membrane protein with a molecular weight of 116,000. Mutants in traG (or in traN) are able to trigger conjugal DNA replication even though they cannot efficiently form stable mating aggregates with F(-) cells. The traG protein (and the traN protein) probably acts in the donor at the stabilization stage of conjugation.

Entities: Gene Species

Mesh：

Substances：

Year: 1981 PMID： 7038680 PMCID： PMC349293 DOI： 10.1073/pnas.78.12.7487

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Operon structure of DNA transfer cistrons on the F sex factor.

Authors: R Helmuth; M Achtman
Journal: Nature Date: 1975-10-23 Impact factor: 49.962

2. Mating aggregates in Escherichia coli conjugation.

Authors: M Achtman
Journal: J Bacteriol Date: 1975-08 Impact factor: 3.490

3. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.

Authors: J P Rosenbusch
Journal: J Biol Chem Date: 1974-12-25 Impact factor: 5.157

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors: P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal: J Mol Biol Date: 1972-11-14 Impact factor: 5.469

5. Cell envelope and shape of Escherichia coli K12. The ghost membrane.

Authors: U Henning; B Höhn; I Sonntag
Journal: Eur J Biochem Date: 1973-11-01

6. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.

Authors: M Achtman; N Willetts; A J Clark
Journal: J Bacteriol Date: 1972-06 Impact factor: 3.490

7. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.

Authors: N Willetts; M Achtman
Journal: J Bacteriol Date: 1972-06 Impact factor: 3.490

8. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors: M J Osborn; J E Gander; E Parisi; J Carson
Journal: J Biol Chem Date: 1972-06-25 Impact factor: 5.157

9. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.

Authors: M Achtman; N Willetts; A J Clark
Journal: J Bacteriol Date: 1971-05 Impact factor: 3.490

10. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.

Authors: C A Schnaitman
Journal: J Bacteriol Date: 1971-10 Impact factor: 3.490

24 in total

1. Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation.

Authors: K G Anthony; W A Klimke; J Manchak; L S Frost
Journal: J Bacteriol Date: 1999-09 Impact factor: 3.490

9. The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.

Authors: A Darzins
Journal: J Bacteriol Date: 1993-09 Impact factor: 3.490

10. The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.

Authors: D Moore; C M Hamilton; K Maneewannakul; Y Mintz; L S Frost; K Ippen-Ihler
Journal: J Bacteriol Date: 1993-03 Impact factor: 3.490

traG protein of the F sex factor of Escherichia coli K-12 and its role in conjugation.

1. Operon structure of DNA transfer cistrons on the F sex factor.

2. Mating aggregates in Escherichia coli conjugation.

3. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.

4. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

5. Cell envelope and shape of Escherichia coli K12. The ghost membrane.

6. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.

7. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.

8. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

9. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.

10. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.

1. Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation.

2. Localization of TraC, a protein involved in assembly of the F conjugative pilus.

3. Determinants of entry exclusion within Eex and TraG are cytoplasmic.

4. F plasmid TraF and TraH are components of an outer membrane complex involved in conjugation.

5. The product of the F plasmid transfer operon gene, traF, is a periplasmic protein.

6. Characterization of the F-plasmid conjugative transfer gene traU.

7. Selection and Plasmid Transfer Underlie Adaptive Mutation in Escherichia coli.

8. Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.

9. The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.

10. The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.