Literature DB >> 7037808

A method for recovering viruses from sludges.

D Berman, G Berg, R S Safferman.   

Abstract

Primary, activated, and anaerobic mesophilically digested sludges were salted with MgCl2 (divalent cations) or AlCl3 (trivalent cations) and acidified to bind indigenous unadsorbed virions to the sludge solids; the sludges were centrifuged, and the adsorbed virions were eluted from the solids with buffered 10% beef extract. The elution yields with this procedure were superior to those obtained from sludges that had been salted or acidified only. Homogenization of sludges prior to other treatment did not increase the numbers of virions recovered.

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Year:  1981        PMID: 7037808     DOI: 10.1016/0166-0934(81)90066-5

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  8 in total

1.  Optimum pH levels for eluting enteroviruses from sludge solids with beef extract.

Authors:  G Berg; G Sullivan
Journal:  Appl Environ Microbiol       Date:  1988-07       Impact factor: 4.792

2.  Enteroviruses in sludge: multiyear experience with four wastewater treatment plants.

Authors:  V V Hamparian; A C Ottolenghi; J H Hughes
Journal:  Appl Environ Microbiol       Date:  1985-08       Impact factor: 4.792

3.  EU policy on sewage sludge utilization and perspectives on new approaches of sludge management.

Authors:  G Mininni; A R Blanch; F Lucena; S Berselli
Journal:  Environ Sci Pollut Res Int       Date:  2014-06-20       Impact factor: 4.223

4.  Low-temperature stability of viruses in sludges.

Authors:  G Berg; G Sullivan; A D Venosa
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

5.  Assessment of recovery efficiency of beef extract reagents for concentrating viruses from municipal wastewater sludge solids by the organic flocculation procedure.

Authors:  R S Safferman; M E Rohr; T Goyke
Journal:  Appl Environ Microbiol       Date:  1988-02       Impact factor: 4.792

6.  Reduction of interfering cytotoxicity associated with wastewater sludge concentrates assayed for indigenous enteric viruses.

Authors:  C J Hurst; T Goyke
Journal:  Appl Environ Microbiol       Date:  1983-07       Impact factor: 4.792

7.  Use of bituminous coal as an alternative technique for field concentration of waterborne viruses.

Authors:  D R Dahling; P M Phirke; B A Wright; R S Safferman
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

8.  Comparison of methods for recovering indigenous viruses from raw wastewater sludge.

Authors:  D A Brashear; R L Ward
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

  8 in total

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