Literature DB >> 7037614

Comparison of a simplified whole blood and isolated lymphocyte stimulation technique.

S E Keller, S J Schleifer, J Sherman, M S Camerino, H Smith, M Stein.   

Abstract

Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocytes stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with 3H-thymidine were comparable to responses utilizing 125IUdR.

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Year:  1981        PMID: 7037614     DOI: 10.3109/08820138109050707

Source DB:  PubMed          Journal:  Immunol Commun        ISSN: 0090-0877


  3 in total

1.  Immune function in healthy inner-city children.

Authors:  J A Bartlett; A R Goldklang; S J Schleifer; S E Keller
Journal:  Clin Diagn Lab Immunol       Date:  2001-07

2.  Immune function in healthy adolescents.

Authors:  J A Bartlett; S J Schleifer; M K Demetrikopoulos; B R Delaney; S C Shiflett; S E Keller
Journal:  Clin Diagn Lab Immunol       Date:  1998-01

3.  Leukocytosis and natural killer cell function parallel neurobehavioral fatigue induced by 64 hours of sleep deprivation.

Authors:  D F Dinges; S D Douglas; L Zaugg; D E Campbell; J M McMann; W G Whitehouse; E C Orne; S C Kapoor; E Icaza; M T Orne
Journal:  J Clin Invest       Date:  1994-05       Impact factor: 14.808

  3 in total

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