Literature DB >> 7036343

Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins.

D C Williams, R M Van Frank, W L Muth, J P Burnett.   

Abstract

Escherichia coli that has been genetically manipulated by recombinant DNA technology to synthesize human insulin polypeptides (A chain, B chain, or proinsulin) contains prominent cytoplasmic inclusion bodies. The amount of inclusion product within the cells corresponds to the quantity of chimeric protein formed by the bacteria. At peak production, the inclusion bodies may occupy as much as 20 percent of the Escherichia coli cellular volume.

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Year:  1982        PMID： 7036343     DOI： 10.1126/science.7036343

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  67 in total

1.  Protein compositional analysis of inclusion bodies produced in recombinant Escherichia coli.

Authors:  U Rinas; J E Bailey
Journal:  Appl Microbiol Biotechnol       Date:  1992-08       Impact factor: 4.813

2.  N-terminal domain of the V-ATPase a2-subunit displays integral membrane protein properties.

Authors:  Maria Merkulova; Mary McKee; Phat Vinh Dip; Gerhard Grüber; Vladimir Marshansky
Journal:  Protein Sci       Date:  2010-10       Impact factor: 6.725

Review 3.  Stability of protein pharmaceuticals.

Authors:  M C Manning; K Patel; R T Borchardt
Journal:  Pharm Res       Date:  1989-11       Impact factor: 4.200

4.  Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli.

Authors:  A K Dubey; B Mollet; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

5.  Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system.

Authors:  H B Kaplan; E P Greenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

6.  Large-scale preparation of ribulosebisphosphate carboxylase from a recombinant system in Escherichia coli characterized by extreme plasmid instability.

Authors:  J Pierce; S Gutteridge
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

7.  Sequence and structure of the Drosophila melanogaster ovarian tumor gene and generation of an antibody specific for the ovarian tumor protein.

Authors:  W R Steinhauer; R C Walsh; L J Kalfayan
Journal:  Mol Cell Biol       Date:  1989-12       Impact factor: 4.272

8.  Construction of a model secretion system for oral streptococci.

Authors:  T Shiroza; H K Kuramitsu
Journal:  Infect Immun       Date:  1993-09       Impact factor: 3.441

9.  Localization of inclusion bodies in Escherichia coli overproducing beta-lactamase or alkaline phosphatase.

Authors:  G Georgiou; J N Telford; M L Shuler; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

10.  Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.

Authors:  M A Boss; J H Kenten; C R Wood; J S Emtage
Journal:  Nucleic Acids Res       Date:  1984-05-11       Impact factor: 16.971
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