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Literature DB >> 7032454

OKT 6-positive cells: their demonstration in human thymus, and the effect of fixation on the immunoperoxidase reaction.

Abstract

Human thymocytes at an intermediate stage of differentiation were demonstrated in situ in tissue sections of thymus using the mouse monoclonal antibody OKT 6 THY (Ortho Immunobiology Ltd, Raritan, NJ) and the immunoperoxidase technique. These cells were found to reside mainly within the thymic cortex. The effects of on-slide fixation on the ease of demonstration of the antigen involved were assessed. The choice of fixative was found to have a marked effect, acetone and paraformaldehyde producing the most satisfactory results.

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Year: 1982 PMID： 7032454

Source DB: PubMed Journal: Arch Pathol Lab Med ISSN： 0003-9985 Impact factor: 5.534

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Cited

8 in total

1. The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining.

Authors: Y Sato; K Mukai; S Watanabe; M Goto; Y Shimosato
Journal: Am J Pathol Date: 1986-12 Impact factor: 4.307

2. Differences in marker expression among branched histiocytic cells in T-cell areas of the lymphoreticular system and among their epidermis- and mucosa-associated equivalents.

Authors: G Mechtersheimer; I Brandt; P Möller
Journal: Cell Tissue Res Date: 1986 Impact factor: 5.249

3. Combined immunohistochemical staining for surface IgD and T-lymphocyte subsets with monoclonal antibodies in human tonsils.

Authors: A C Feller; M R Parwaresch; H H Wacker; H J Radzun; K Lennert
Journal: Histochem J Date: 1983-06

8. An improved method for the inhibition of endogenous peroxidase non-deleterious to lymphocyte surface markers. Application to immunoperoxidase studies on eosinophil-rich tissue preparations.

Authors: S M Andrew; B Jasani
Journal: Histochem J Date: 1987-08

8 in total

OKT 6-positive cells: their demonstration in human thymus, and the effect of fixation on the immunoperoxidase reaction.

1. The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining.

2. Differences in marker expression among branched histiocytic cells in T-cell areas of the lymphoreticular system and among their epidermis- and mucosa-associated equivalents.

3. Combined immunohistochemical staining for surface IgD and T-lymphocyte subsets with monoclonal antibodies in human tonsils.

4. Lymphocytes and Langerhans cells in the human oesophageal epithelium.

5. Demonstration of cells bearing the OKT6 determinant in human tonsil and lymph node.

6. Immunohistochemical localization and distribution of S-100 proteins in the human lymphoreticular system.

7. Loss of antibody binding to prefixed cells: fixation parameters for immunocytochemistry.

8. An improved method for the inhibition of endogenous peroxidase non-deleterious to lymphocyte surface markers. Application to immunoperoxidase studies on eosinophil-rich tissue preparations.