Literature DB >> 7031657

Arrangement of the subunits in the ribosome of Escherichia coli: demonstration by immunoelectron microscopy.

B Kastner, M Stöffler-Meilicke, G Stöffler.   

Abstract

The three-dimensional locations of Escherichia coli ribosomal proteins S13, L1, and L7/L12 on the surface of ribosomal subunits and 70S monomeric ribosomes were determined by electron microscopy of antibody-labeled ribosomal particles. A new approach to orient the subunits within 70S ribosomes was developed that used 30S.70S.50S triples that were prepared by simultaneous combination with one antibody directed against a 30S protein and another directed against a 50S protein. Electron microscope studies of triples obtained with the antibody combinations anti-S13/anti-L1 and anti-S13/anti-L7/L12 showed that, in 70S monomeric ribosomes, the head of the 30S subunit is proximate to protein L1 and the peptidyl transferase center but far from the rod-like appendage containing proteins L7 and L12.

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Year:  1981        PMID： 7031657      PMCID： PMC349107          DOI： 10.1073/pnas.78.11.6652

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Structural dynamics of bacterial ribosomes. I. Characterization of vacant couples and their relation to complexed ribosomes.

Authors:  M Noll; B Hapke; M H Schreier; H Noll
Journal:  J Mol Biol       Date:  1973-04-05       Impact factor: 5.469

2.  Location of proteins S5, S13 and S14 on the surface of the 3oS ribosomal subunit from Escherichia coli as determined by immune electron microscopy.

Authors:  G W Tischendorf; H Zeichhardt; G Stöffler
Journal:  Mol Gen Genet       Date:  1974

3.  Determination of the location of proteins L14, L17, L18, L19, L22, L23 on the surface of the 5oS ribosomal subunit of Escherichia coli by immune electron microscopy.

Authors:  G W Tischendorf; H Zeichhardt; G Stöffler
Journal:  Mol Gen Genet       Date:  1974

4.  Electron microscopic localization of two proteins on the surface of the 50 S ribosomal subunit of Escherichia coli using specific antibody markers.

Authors:  M R Wabl
Journal:  J Mol Biol       Date:  1974-04-05       Impact factor: 5.469

5.  The accessibility of proteins of the Escherichia coli 30S ribosomal subunit to antibody binding.

Authors:  G Stöffler; R Hasenbank; M Lütgehaus; R Maschler; C A Morrison; H Zeichhardt; R A Garrett
Journal:  Mol Gen Genet       Date:  1973-12-20

6.  Observations on the shape of the 50S ribosomal subunit.

Authors:  M Lubin
Journal:  Proc Natl Acad Sci U S A       Date:  1968-12       Impact factor: 11.205

7.  Architecture of the Escherichia coli ribosome as determined by immune electron microscopy.

Authors:  G W Tischendorf; H Zeichhardt; G Stöffler
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

8.  Mutants of Escherichia coli lacking ribosomal protein L1.

Authors:  E R Dabbs; R Ehrlich; R Hasenbank; B H Schroeter; M Stöffler-Meilicke; G Stöffler
Journal:  J Mol Biol       Date:  1981-07-15       Impact factor: 5.469

9.  Morphology of the ribosomal 30S subparticle according to electron microscopic data.

Authors:  V D Vasiliev
Journal:  Acta Biol Med Ger       Date:  1974

10.  Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins.

Authors:  G Stöffler; H G Wittmann
Journal:  J Mol Biol       Date:  1971-12-14       Impact factor: 5.469
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  4 in total

1.  Sequence alignment and evolutionary comparison of the L10 equivalent and L12 equivalent ribosomal proteins from archaebacteria, eubacteria, and eucaryotes.

Authors:  L C Shimmin; C Ramirez; A T Matheson; P P Dennis
Journal:  J Mol Evol       Date:  1989-11       Impact factor: 2.395

2.  Characterisation of a mutant from Escherichia coli lacking protein L15 and localisation of protein L15 by immuno-electron microscopy.

Authors:  M Lotti; E R Dabbs; R Hasenbank; M Stöffler-Meilicke; G Stöffler
Journal:  Mol Gen Genet       Date:  1983

3.  Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli.

Authors:  M Stöffler-Meilicke; M Noah; G Stöffler
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

4.  Localization of the puromycin binding site on the large ribosomal subunit of Escherichia coli by immunoelectron microscopy.

Authors:  R Lührmann; R Bald; M Stöffler-Meilicke; G Stöffler
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205
  4 in total

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