Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A minimal mechanism for abortive initiation of transcription of T7 DNA.

Literature DB >> 7031607

A minimal mechanism for abortive initiation of transcription of T7 DNA.

Abstract

A steady state kinetic analysis of product inhibition in abortive initiation of transcription at promoters A1 and A3 of T7 DNA was carried out. The obtained kinetic data are in agreement with a mechanism of ordered product release with pppApU being released first from the central complex. A minimal general mechanism for abortive initiation satisfying the kinetic results is given. The stimulation of transcription of T7 DNA by pppApU at low substrate concentrations was investigated.

Mesh：

Substances：

Year: 1981 PMID： 7031607 PMCID： PMC327564 DOI： 10.1093/nar/9.21.5845

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

1. On the mechanism of rifampicin inhibition of RNA synthesis.

Authors: W R McClure; C L Cech
Journal: J Biol Chem Date: 1978-12-25 Impact factor: 5.157

2. A steady state assay for the RNA polymerase initiation reaction.

Authors: W R McClure; C L Cech; D E Johnston
Journal: J Biol Chem Date: 1978-12-25 Impact factor: 5.157

3. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.

Authors: W F Mangel; M J Chamberlin
Journal: J Biol Chem Date: 1974-05-25 Impact factor: 5.157

3 in total

A minimal mechanism for abortive initiation of transcription of T7 DNA.

1. On the mechanism of rifampicin inhibition of RNA synthesis.

2. A steady state assay for the RNA polymerase initiation reaction.

3. Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.

4. The stereochemical basis of template function.

5. Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.

6. Primed abortive initiation of RNA synthesis by E. coli RNA polymerase on T7 DNA. Steady state kinetic studies.

1. A new way to start: nanoRNA-mediated priming of transcription initiation.

2. NanoRNAs: a class of small RNAs that can prime transcription initiation in bacteria.

3. NanoRNAs prime transcription initiation in vivo.