Literature DB >> 7031067

Freeze-fracture cytochemistry: localization of wheat-germ agglutinin and concanavalin A binding sites on freeze-fractured pancreatic cells.

P Pinto da Silva, M R Torrisi, B Kachar.   

Abstract

The combined application of thin-section and critical-point-drying "fracture-label" is used to determine the pattern of distribution and partition of wheat-germ agglutinin and concanavalin A binding sites on the membrane faces of freeze-fractured exocrine and endocrine rat pancreatic cells. Whereas the exoplasmic face of plasma membrane is preferentially labeled by both lectins, the endoplasmic reticulum and nuclear envelope are strongly and uniformly labeled by concanavalin A but not by wheat-germ agglutinin. The results support current views in the glycosylation of membrane proteins and do not support the backflow of sialidated glycoproteins to the endoplasmic reticulum.

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Year:  1981        PMID： 7031067      PMCID： PMC2111992          DOI： 10.1083/jcb.91.2.361

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  25 in total

1.  Distribution of protein-bound sugar residues in microsomal subfractions and Golgi membranes.

Authors:  A Bergman; G Dallner
Journal:  Biochim Biophys Acta       Date:  1976-05-21

2.  Biogenesis of microsomal membrane glycoproteins in rat liver. IV. Characteristics of a cytoplasmic lipoprotein having properties of a membrane precursor.

Authors:  H Svensson; A Elhammer; F Autuori; G Dallner
Journal:  Biochim Biophys Acta       Date:  1976-12-02

3.  Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.

Authors:  W D Geoghegan; G A Ackerman
Journal:  J Histochem Cytochem       Date:  1977-11       Impact factor: 2.479

4.  Colloidal gold, a useful marker for transmission and scanning electron microscopy.

Authors:  M Horisberger; J Rosset
Journal:  J Histochem Cytochem       Date:  1977-04       Impact factor: 2.479

5.  Fracture faces of frozen membranes.

Authors:  D Branton
Journal:  Proc Natl Acad Sci U S A       Date:  1966-05       Impact factor: 11.205

6.  Extensive binding of concanavalin A to the nuclear membrane.

Authors:  A Monneron; D Segretain
Journal:  FEBS Lett       Date:  1974-06-01       Impact factor: 4.124

7.  Ganglioside biosynthesis. Characterization of CMP-N-acetylneuraminic acid : lactosylceramide sialyltransferase in Golgi apparatus from rat liver.

Authors:  C L Richardson; T W Keenan; D J Morre
Journal:  Biochim Biophys Acta       Date:  1977-07-20

8.  Freeze-fracture cytochemistry: thin sections of cells and tissues after labeling of fractures faces.

Authors:  P P da Silva; C Parkison; N Dwyer
Journal:  J Histochem Cytochem       Date:  1981-08       Impact factor: 2.479

9.  Lectin receptor sites on rat liver cell nuclear membranes.

Authors:  I Virtanen; J Wartiovaara
Journal:  J Cell Sci       Date:  1976-11       Impact factor: 5.285

10.  Biogenesis of microsomal membrane glycoproteins in rat liver. III. Release of glycoproteins from the Golgi fraction and their transfer to microsomal membranes.

Authors:  A Elhammer; H Svensson; F Autuori; G Dallner
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539
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  20 in total

1.  Herpes simplex virus envelopment and maturation studied by fracture label.

Authors:  M R Torrisi; C Di Lazzaro; A Pavan; L Pereira; G Campadelli-Fiume
Journal:  J Virol       Date:  1992-01       Impact factor: 5.103

Review 2.  The silver anniversary of gold: 25 years of the colloidal gold marker system for immunocytochemistry and histochemistry.

Authors:  J Roth
Journal:  Histochem Cell Biol       Date:  1996-07       Impact factor: 4.304

3.  Viral glycoproteins accumulate in newly formed annulate lamellae following infection of lymphoid cells by human herpesvirus 6.

Authors:  G Cardinali; M Gentile; M Cirone; C Zompetta; L Frati; A Faggioni; M R Torrisi
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103

4.  Translocation and compartmentalization of Escherichia coli hemolysin (HlyA).

Authors:  R L Oropeza-Wekerle; W Speth; B Imhof; I Gentschev; W Goebel
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

5.  Glycoconjugate pattern of membranes in the acinar cell of the rat pancreas.

Authors:  S Willemer; H Köhler; R Naumann; H F Kern; G Adler
Journal:  Histochemistry       Date:  1990

6.  Ultrastructural visualisation of carbohydrate groups in the surface coating of hamster alveolar macrophages and pneumonocytes.

Authors:  C Meban
Journal:  J Anat       Date:  1986-06       Impact factor: 2.610

7.  Freeze-fracture cytochemistry of wheat germ agglutinin and concanavalin A receptors on the plasma membrane of normal, Bernard-Soulier, and thrombasthenic platelets.

Authors:  J Chevalier; J P Caen; P Pinto da Silva
Journal:  Am J Pathol       Date:  1986-02       Impact factor: 4.307

8.  The herpes simplex virus UL20 protein compensates for the differential disruption of exocytosis of virions and viral membrane glycoproteins associated with fragmentation of the Golgi apparatus.

Authors:  E Avitabile; P L Ward; C Di Lazzaro; M R Torrisi; B Roizman; G Campadelli-Fiume
Journal:  J Virol       Date:  1994-11       Impact factor: 5.103

9.  The cellular specificity of lectin binding in the kidney. II. A light microscopical study in the rabbit.

Authors:  M Le Hir; U C Dubach
Journal:  Histochemistry       Date:  1982

10.  The cellular specificity of lectin binding in the kidney. I. A light microscopical study in the rat.

Authors:  M Le Hir; U C Dubach
Journal:  Histochemistry       Date:  1982
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