Literature DB >> 7030534

The haemolytic antibody isotope release (HAIR) assay: an efficient alternative technique to conventional plaque assays.

L Baecher-Steppan, N I Kerkvliet.   

Abstract

The haemolytic antibody isotope release (HAIR) assay quantitates antibody production by splenic antibody-producing cells by lysis of chromium-51-labelled sheep red blood cells. The amount of antibody quantitated by the HAIR assay directly correlates with the number of antibody-producing cells measured by a conventional plaque assay. The HAIR assay is an easy, sensitive, and reproducible technique that is especially useful when large numbers of animals are required for testing.

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Year:  1981        PMID： 7030534      PMCID： PMC1537334     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  7 in total

1.  Plaque formation in agar by single antibody-producing cells.

Authors:  N K JERNE; A A NORDIN
Journal:  Science       Date:  1963-04-26       Impact factor: 47.728

2.  Murine T cells that lyse antibody-sensitized target cells. I. Kinetics and dose-response studies of control and cortisone-resistant thymocytes.

Authors:  E W Lamon; B R Williams; E W Fuson; H D Whitten; A S Walia
Journal:  J Immunol       Date:  1978-01       Impact factor: 5.422

3.  Further improvements in the plaque technique for detecting single antibody-forming cells.

Authors:  A J Cunningham; A Szenberg
Journal:  Immunology       Date:  1968-04       Impact factor: 7.397

4.  Cellular assay for measuring anti-erythrocyte antibody responses.

Authors:  B Leshem; D Naor
Journal:  J Immunol Methods       Date:  1978       Impact factor: 2.303

5.  A novel technique to measure total IgM and IgG in vitro haemolysin production by mouse spleen cells, using 51Cr-labelled sheep red blood cells.

Authors:  R Liske
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

6.  A method of increased sensitivity for detecting single antibody-forming cells.

Authors:  A J Cunningham
Journal:  Nature       Date:  1965-09-04       Impact factor: 49.962

7.  Synergism of methylmercury and selenium producing enhanced antibody formation in mice.

Authors:  L D Koller; N Isaacson-Kerkvliet; J H Exon; J A Brauner; N M Patton
Journal:  Arch Environ Health       Date:  1979 Jul-Aug
  7 in total

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