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Literature DB >> 7030322

Characterization of human muscle myosins with respect to the light chains.

P Volpe, D Biral, E Damiani, A Margreth.

Abstract

Isolated myosins from human predominantly fast and slow muscles, human neonatal and foetal muscle were examined for light chain composition by one- and two-dimensional electrophoresis. The LC1F, LC2F and LC3F light chains were identical with their counterparts from rabbit fast myosin. Human LC1S was identified by correlative criteria as a single component having a molecular weight slightly lower than, but an electric charge similar to, that of rabbit LC1Sb. Consequently, human LC1S appears to be much less heterogeneous relative to LC1F than is the case with other mammalian species. A high immunological cross-reactivity was likewise observed, with antibody specific to rabbit LC1F, between the isolated myosins from several human mixed muscles and rabbit fast myosin, though reactivity was highest with foetal myosin (having a pure-fast-light-chain pattern).

Entities: Disease Species

Mesh：

Substances：
Peptide Fragments
Myosins

Year: 1981 PMID： 7030322 PMCID： PMC1162879 DOI： 10.1042/bj1950251

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

29 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

3. Data on the distribution of fibre types in thirty-six human muscles. An autopsy study.

Authors: M A Johnson; J Polgar; D Weightman; D Appleton
Journal: J Neurol Sci Date: 1973-01 Impact factor: 3.181

4. Rise time of the spike potential in fast and slowly contracting muscle of man.

Authors: F Buchthal; K Dahl; P Rosenfalck
Journal: Acta Physiol Scand Date: 1973-02

5. A phosphorylated light-chain component of myosin from skeletal muscle.

Authors: W T Perrie; L B Smillie; S B Perry
Journal: Biochem J Date: 1973-09 Impact factor: 3.857

6. Slow and fast twitch fibers in human skeletal muscle.

Authors: A Eberstein; J Goodgold
Journal: Am J Physiol Date: 1968-09

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

8. Contraction times and fibre types in intact human muscle.

Authors: F Buchthal; H Schmalbruch
Journal: Acta Physiol Scand Date: 1970-08

9. An immunological approach to the role of the low molecular weight subunits in myosin. II. Interaction of myosin and its subfragments with antibodies to the light chains.

Authors: J C Holt; S Lowey
Journal: Biochemistry Date: 1975-10-21 Impact factor: 3.162

10. The transformation of myosin in cross-innervated rat muscles.

Authors: M Bárány; R I Close
Journal: J Physiol Date: 1971-03 Impact factor: 5.182

13 in total

1. Transitions in membrane composition during postnatal development of rabbit fast muscle.

Authors: P Volpe; E Damiani; G Salviati; A Margreth
Journal: J Muscle Res Cell Motil Date: 1982-06 Impact factor: 2.698

2. Biochemical heterogeneity of skeletal-muscle microsomal membranes. Membrane origin, membrane specificity and fibre types.

Authors: G Salviati; P Volpe; S Salvatori; R Betto; E Damiani; A Margreth; I Pasquali-Ronchetti
Journal: Biochem J Date: 1982-02-15 Impact factor: 3.857

Characterization of human muscle myosins with respect to the light chains.

1. Protein measurement with the Folin phenol reagent.

2. High resolution two-dimensional electrophoresis of proteins.

3. Data on the distribution of fibre types in thirty-six human muscles. An autopsy study.

4. Rise time of the spike potential in fast and slowly contracting muscle of man.

5. A phosphorylated light-chain component of myosin from skeletal muscle.

6. Slow and fast twitch fibers in human skeletal muscle.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

8. Contraction times and fibre types in intact human muscle.

9. An immunological approach to the role of the low molecular weight subunits in myosin. II. Interaction of myosin and its subfragments with antibodies to the light chains.

10. The transformation of myosin in cross-innervated rat muscles.

1. Transitions in membrane composition during postnatal development of rabbit fast muscle.

2. Biochemical heterogeneity of skeletal-muscle microsomal membranes. Membrane origin, membrane specificity and fibre types.

3. Myofibrillar-protein isoforms and sarcoplasmic-reticulum Ca2+-transport activity of single human muscle fibres.

4. Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle.

5. Myosin light chain phosphorylation and isometric twitch potentiation in intact human muscle.

6. Expression of myosin light chains during fetal development of human skeletal muscle.

7. Characteristics of skeletal muscle calsequestrin: comparison of mammalian, amphibian and avian muscles.

8. Polymorphism of myofibrillar proteins of rabbit skeletal-muscle fibres. An electrophoretic study of single fibres.

9. Polymorphism of myosin light chains. An electrophoretic and immunological study of rabbit skeletal-muscle myosins.

10. Myosin subunit composition in human developing muscle.