Literature DB >> 7029471

Studies on the selectivity of DNA precipitation by spermine.

B C Hoopes, W R McClure.   

Abstract

We have examined the selectivity of the precipitation of DNA by spermine. We have found that the intra- and intermolecular condensation of DNA induced by spermine is highly selective even in the presence of added protein or triphosphates. We have also investigated the influence of buffer components on the threshold concentration of spermine required for DNA precipitation. Representative applications exploiting the selectivity of the precipitation reaction are also described.

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Year:  1981        PMID: 7029471      PMCID: PMC327535          DOI: 10.1093/nar/9.20.5493

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  11 in total

1.  Spermine protection of coliphage lambda DNA against breakage by hydrodynamic shear.

Authors:  D KAISER; H TABOR; C W TABOR
Journal:  J Mol Biol       Date:  1963-02       Impact factor: 5.469

2.  Compact form of DNA induced by spermidine.

Authors:  L C Gosule; J A Schellman
Journal:  Nature       Date:  1976-01-29       Impact factor: 49.962

3.  A steady state assay for the RNA polymerase initiation reaction.

Authors:  W R McClure; C L Cech; D E Johnston
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

Review 4.  The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides.

Authors:  G S Manning
Journal:  Q Rev Biophys       Date:  1978-05       Impact factor: 5.318

5.  The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid.

Authors:  G M Rubin
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

6.  Counterion-induced condesation of deoxyribonucleic acid. a light-scattering study.

Authors:  R W Wilson; V A Bloomfield
Journal:  Biochemistry       Date:  1979-05-29       Impact factor: 3.162

7.  Determination of DNA composition and concentration by spectral analysis.

Authors:  S Z Hirschman; G Felsenfeld
Journal:  J Mol Biol       Date:  1966-04       Impact factor: 5.469

8.  DNA condensation with polyamines I. Spectroscopic studies.

Authors:  L C Gosule; J A Schellman
Journal:  J Mol Biol       Date:  1978-05-25       Impact factor: 5.469

9.  DNA condensation with polyamines. II. Electron microscopic studies.

Authors:  D K Chattoraj; L C Gosule; A Schellman
Journal:  J Mol Biol       Date:  1978-05-25       Impact factor: 5.469

10.  Cation-induced toroidal condensation of DNA studies with Co3+(NH3)6.

Authors:  J Widom; R L Baldwin
Journal:  J Mol Biol       Date:  1980-12-25       Impact factor: 5.469

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  92 in total

1.  A method for preparing genomic DNA that restrains branch migration of Holliday junctions.

Authors:  T Allers; M Lichten
Journal:  Nucleic Acids Res       Date:  2000-01-15       Impact factor: 16.971

2.  Development of an in vitro integration assay for the Bacteroides conjugative transposon CTnDOT.

Authors:  Qi Cheng; Neil Wesslund; Nadja B Shoemaker; Abigail A Salyers; Jeffrey F Gardner
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

3.  DNA Methylation is Reduced in DNasel-Sensitive Regions of Plant Chromatin.

Authors:  M Klaas; R M Amasino
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

4.  Cloning in Escherichia coli of a Bacillus subtilis arginine repressor gene through its ability to confer structural stability on a fragment carrying genes of arginine biosynthesis.

Authors:  M C Smith; A Mountain; S Baumberg
Journal:  Mol Gen Genet       Date:  1986-10

5.  Genetic and molecular analysis of vgU and vgW: two dominant vg alleles associated with gene fusions in Drosophila.

Authors:  J A Williams; I M Scott; A L Atkin; W J Brook; M A Russell; J B Bell
Journal:  Genetics       Date:  1990-08       Impact factor: 4.562

6.  Type I and type II genes for the chlorophyll a/b-binding protein in the gymnosperm Pinus sylvestris (Scots pine): cDNA cloning and sequence analysis.

Authors:  S Jansson; P Gustafsson
Journal:  Plant Mol Biol       Date:  1990-03       Impact factor: 4.076

7.  Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ.

Authors:  T Linn; R St Pierre
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

8.  Sequence-dependent DNA condensation as a driving force of DNA phase separation.

Authors:  Hyunju Kang; Jejoong Yoo; Byeong-Kwon Sohn; Seung-Won Lee; Hong Soo Lee; Wenjie Ma; Jung-Min Kee; Aleksei Aksimentiev; Hajin Kim
Journal:  Nucleic Acids Res       Date:  2018-10-12       Impact factor: 16.971

9.  Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.

Authors:  S J Elledge; J T Mulligan; S W Ramer; M Spottswood; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

10.  Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice.

Authors:  D E Graham; D Medina; G H Smith
Journal:  J Virol       Date:  1984-03       Impact factor: 5.103

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