Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Regular segregation of composite plasmid Rms201.

Literature DB >> 7028717

Regular segregation of composite plasmid Rms201.

Abstract

Copy number mutants Rms201ts15 and Rms201ts16 were isolated at 30 degrees C from a temperature-sensitive replication mutant (Rms201ts14) of the conjugative plasmid Rms201. The numbers of plasmids per chromosome of ML1410(Rms201ts14), ML1410(Rms201ts15), and ML1410(Rms201ts16) grown at 30 degrees C were 2.2, 7.4, and 20, respectively. The synthesis of covalently closed circular plasmid deoxyribonucleic acid stopped in Rms201ts14, Rms201ts15, and Rms201ts16 immediately after a "shift-up" in temperature (42 degrees C). At 42 degrees C, antibiotic-sensitive derivatives appeared after a certain lag time: the lag times of ML1410(Rms201ts14), ML1410(Rms201ts15), and ML1410(Rms201ts16) were 2.5, 5, and 6.8 generations, respectively. After these times, plasmid-positive cells in the populations decreased at a rate of about 50% per generation in all of the mutants. From these results we conclude that plasmid segregation (partition) of Rms201 occurs by regular segregation (partition).

Mesh：

Substances：
DNA, Circular
Ampicillin

Year: 1981 PMID： 7028717 PMCID： PMC216237 DOI： 10.1128/jb.148.2.534-540.1981

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

23 in total

1. In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities.

Authors: K Matsubara; Y Takagi; T Mukai
Journal: J Virol Date: 1975-09 Impact factor: 5.103

2. Mutants of Escherichia coli requiring methionine or vitamin B12.

Authors: B D DAVIS; E S MINGIOLI
Journal: J Bacteriol Date: 1950-07 Impact factor: 3.490

3. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.

Authors: P A Sharp; S N Cohen; N Davidson
Journal: J Mol Biol Date: 1973-04-05 Impact factor: 5.469

4. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.

Authors: Y M Kupersztoch; D R Helinski
Journal: Biochem Biophys Res Commun Date: 1973-10-15 Impact factor: 3.575

3. Temperature sensitive replication plasmids are passively distributed during cell division at non-permissive temperature: a new model for replicon duplication and partitioning.

Authors: T Hashimoto-Gotoh; K Ishii
Journal: Mol Gen Genet Date: 1982

3 in total

Regular segregation of composite plasmid Rms201.

1. In vitro construction of different oligomeric forms of lambdadv DNA and studies of their transforming activities.

2. Mutants of Escherichia coli requiring methionine or vitamin B12.

3. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.

4. A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.

5. Ampicillin-resistant R factors derived from Shigella strains.

6. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

7. Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.

8. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].

9. Isolation and characterization of a copy mutant of the bacteriocinogenic plasmid Clo DF13.

10. Round of replication mutant of a drug resistance factor.

1. Partition of nonreplicating DNA by the par system of bacteriophage P1.

2. Equipartition and other modes of partition: on the interpretation of curing kinetics using rep(ts) plasmids.

3. Temperature sensitive replication plasmids are passively distributed during cell division at non-permissive temperature: a new model for replicon duplication and partitioning.