Literature DB >> 7026974

Initiation of chromosomal DNA synthesis in vitro.

S J Projan, J A Wechsler.   

Abstract

An in vitro complementation assay for initiation of chromosomal DNA replication is described. The initiation reaction is dependent upon extract from either of two hybrid-plasmid containing strains. Each hybrid plasmid carries a suppressor of dna A-ts mutations. The in vitro DNA synthesis is heavily biased toward the origin region, and the origin of replication (oriC) is replicated as determined by DNA-DNA hybridizations.

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Year:  1981        PMID： 7026974     DOI： 10.1007/bf00269668

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  17 in total

1.  Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.

Authors:  F G Hansen; K von Meyenburg
Journal:  Mol Gen Genet       Date:  1979-09

2.  A DNA fragment containing the origin of replication of the Escherichia coli chromosome.

Authors:  R C Marsh; A Worcel
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

3.  In vitro replication of a DNA fragment containing the vicinity of the origin of E. coli DNA replication.

Authors:  V Nüsslein-Crystalla; U Scheefers-Borchel
Journal:  Mol Gen Genet       Date:  1979-01-16

4.  Deoxyribonucleic acid replication in vitro.

Authors:  H Schaller; B Otto; V Nüsslein; J Huf; R Herrmann; F Bonhoeffer
Journal:  J Mol Biol       Date:  1972-01-28       Impact factor: 5.469

5.  Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.

Authors:  J A Wechsler; V Nüsslein; B Otto; A Klein; F Bonhoeffer; R Herrmann; L Gloger; H Schaller
Journal:  J Bacteriol       Date:  1973-03       Impact factor: 3.490

6.  Isolation and analysis of multicopy extragenic suppressors of dnaA mutations.

Authors:  S J Projan; J A Wechsler
Journal:  J Bacteriol       Date:  1981-02       Impact factor: 3.490

Review 7.  Linkage map of Escherichia coli K-12, edition 6.

Authors:  B J Bachmann; K B Low
Journal:  Microbiol Rev       Date:  1980-03

Review 8.  Review lecture on the growth and form of a bacterial cell.

Authors:  R H Pritchard
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1974-02-21       Impact factor: 6.237

9.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

10.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688
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  3 in total

1.  Enzymatic replication of the origin of the Escherichia coli chromosome.

Authors:  R S Fuller; J M Kaguni; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

2.  Complementation of a dnaC initiation defect in vitro.

Authors:  S J Projan; J A Wechsler
Journal:  Mol Gen Genet       Date:  1981

3.  RNA polymerase is required for DNA initiation in vitro.

Authors:  S J Projan; J A Wechsler
Journal:  Mol Gen Genet       Date:  1981
  3 in total

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